不同年龄组女性胚胎的非整倍性分析

Aneuploidy in Embryos from Women of Different Age Groups

目的分析不同年龄组女性胚胎的非整倍性情况。方法收集沈阳市妇婴医院生殖中心患者捐赠用于科学研究的胚胎,根据女方年龄将胚胎分为高龄组(A组,年龄>35岁)和低龄组(C组,年龄≤35岁)。将胚胎培养至囊胚期,取囊胚滋养层细胞活检,检测胚胎的非整倍性。结果共收集捐赠胚胎149枚,其中A组胚胎82枚,C组胚胎67枚;A组活检胚胎48枚,染色体异常32例;C组活检胚胎50枚,染色体异常22例。结论高龄女性胚胎染色体异常率高于低龄女性,为获得健康胚胎,建议高龄女性在行体外受精-胚胎移植(IVF-ET)时,结合植入前遗传学筛查(PGS),形态学不能作为胚胎筛选的唯一标准。

Objective To analyze aneuploidy in embryos from women of different age groups.Methods Embryos donated for scientific research by patients in the Reproductive Center of Shenyang Women’s and Children’s Hospital were examined.The embryos were divided into group A from the advanced age group(>35 years old)and group C from the younger group(≤35 years old).After the embryos were cultured to blastocyst stage,trophoblast cells were collected,and aneuploidy was evaluated.Results In group A,32 of 48 embryos had a chromosomal abnormality;in group C,22 of 50 embryos had a chromosomal abnormality.Conclusion The rate of chromosomal abnormalities was higher in embryos from the advanced age group than in those from the younger group.To obtain healthy embryos,women in the advanced age group should undergo in vitro fertilization and embryo transfer in addition to preimplantation genetic screening.Morphology should not be the only criterion for embryo screening.