植物基因组编辑检测方法

Detection methods of genome editing in plants

以CRISPR/Cas9技术为代表的基因组编辑在生物领域的革命性应用使得生命科学研究迈入新篇章。该技术以其灵活性、易用性且扩展性强等优势,大大加快了基因工程研究,也加速了植物分子育种的步伐。但是,遗传转化过程中产生大量潜在的基因编辑植株,使得早期高通量快速筛选和检测目标编辑植株面临很大挑战。本文综述了近年来植物基因组编辑检测的各种方法,比较了其优缺点和适用范围;同时,还对近几年植物基因组编辑检测方法的发展趋势进行了深入分析和展望,以期对基因组编辑技术在植物中的应用提供参考。

The life science has entered a new chapter with the revolutionary implementation of the CRISPR/Cas9 genome editing technology in various living organisms.With the unique flexibility,feasibility and extendibility,the CRISPR/Cas9 technology greatly accelerates genetic engineering research,as well as plant molecular breeding.However,it has become a challenge to screen for and identify genome-edited plants at early stages in a rapid and high-throughput fashion,due to the massive number of plants produced from transformation process.In this review,we summarize the molecular methods developed in recent years to identify genome-edited plants.We compare their advantages and disadvantages,and the scope of application.In addition,we provide insights of the development trend of detection methods for plant genome editing.This review will serve as a reference for future genome editing research in plants.