柴胡疏肝散精简方对抑郁大鼠中缝核内5-羟色胺及色氨酸羟化酶2的影响

Effects of Simplified Chaihu-Shugan Powder on regulating the 5-hydroxytryptamine and tryptophan hydroxylase 2 in raphe nucleus of depressive rats

目的观察柴胡疏肝散精简方对抑郁大鼠中缝核内5-羟色胺及色氨酸羟化酶2的影响,探讨柴胡疏肝散精简方可能的抗抑郁机制。方法将40只SD雄性大鼠采用随机区组法分为空白组(control)、模型组(model)、氟西汀组(fluoxetine)、柴胡疏肝散精简方(Simplified Chaihu Shugan Powder,SCSP)组,每组10只,腹腔注射利血平建立大鼠抑郁模型,同时各药物组灌胃相应药物,连续14 d。通过行为学实验(糖水偏好实验、强迫游泳实验)评价大鼠抑郁状态,以高效液相色谱-电化学法(high performance liquid chromatography-electrochemical detector,HPLC-ECD)测定中缝核内5-羟色胺(5-hydroxytryptamine,5-HT)的含量,以反转录聚合酶链式反应法(real-time quantitative polymerase chain reaction,RT-PCR)测定中缝核内色氨酸羟化酶2(tryptophan hydroxylase 2,TPH2)的含量。结果与空白组比较,模型组体质量、体质量增量、糖水偏好指数明显降低(P <0. 01),游泳不动时间明显增加(P <0. 01),中缝核内TPH2、5-HT含量明显减少(P <0. 01)。与模型组比较,氟西汀组及SCSP组体质量、体质量增量明显增加(P <0. 01),糖水偏好指数增加(P <0. 05),游泳不动时间明显减少(P <0. 01),中缝核内TPH2、5-HT含量明显增加(P <0. 01)。结论柴胡疏肝散精简方对利血平致抑郁模型大鼠具有一定的抗抑郁作用,与氟西汀效果相当其作用机制可能与增加中缝核内TPH2的含量,促进5-HT的生成有关。

Objective To explore the effect and mechanism of Simplified Chaihu-Shugan Powder(SCSP)on hydroxytryptamine and tryptophan hydroxylase2in raphe nucleus of depressive rats.Methods Forty SD rats were divided into control group,model group,fluoxetine group,and SCSP group by randomized block design,with10rats in every group.Reserpine were intraperitoneal injected for14days to establish depressive models,and then the rats were seperatedly treated with the fluoxetine and SCSP.At the end of the experiment,behavioral experiments(sucrose preference test,forced swimming test)were performed to evaluate the depressive status.The contents5-hydroxytryptamine(5-HT)in raphe nucleus were detected with high performance liquid chromatography-electrochemical detector(HPLC-ECD).The contents of tryptophan hydroxylase2(TPH2)in raphe nucleus were detected with real-time quantitative polymerase chain reaction(RT-PCR).Results Comparing to the control group,the body weight,weight gain,sucrose preference in model group decreased significantly(P<0.01),whilst the immobility time increased significantly(P<0.01).The contents of TPH2and5-HT in raphe nucleus decreased significantly(P<0.01)in model group.Comparing to the model group,the body weight,weight gain in SCSP group increased significantly(P<0.01),the sucrose preference increased(P<0.05),whilst the immobility time in SCSP group decreased significantly(P<0.01).The contents of TPH2and5-HT in raphe nucleus increased significantly(P<0.01)in fluoxetine and SCSP group.Conclusion SCSP showed anti-depression effect on the depressive rats caused by reserpine.It has same effect as fluoxetine.It could increase the content of TPH2in raphe nucleus and promote the generation of5-HT,which may be one of the mechanisms of anti-depression by SCSP.