转录因子DREB1A基因的克隆及植物表达载体的构建

Cloning of Transcription Factor DREB1A Gene and Construction of Plant Expression Vector

研究转录因子DREB1A在植物抗渗透胁迫反应中的作用。根据GenBank中登录的DREB1A基因的序列设计引物,用PCR方法从拟南芥中克隆DREB1A基因,利用基因重组技术成功构建了植物表达载体pCAMB1A1300-DREB1A。该结果为进一步利用DREB1A基因做遗传转化研究奠定了基础。

To research the function of the transcription factor DREB1A in plant tolerance to osmotic stress,a pair of primers was designed according to the sequence of DREB1A gene in GenBank databases.The DREB1A gene was cloned from Arabidopsis thaliarra by the method of PCR.By the method of Recombinant DNA Technology,plant expression vector pCAMB1A1300-DREB1A was successfully constructed.The results laid a foundation for further research on genetic transformation using DREB1A gene.