生防放线菌ZZ-9对4种杀菌剂及温度的敏感性测定

Sensitivity of Biocontrol Actinomycete ZZ-9 to 4 Fungicides and Temperatures

采用菌丝生长速率法,分别测定放线菌ZZ-9菌株对不同温度及不同药剂的敏感度,旨在为后期生物制剂的研究提供理论依据。结果表明,放线菌ZZ-9对供试4种杀菌剂均表现出一定的敏感性。其中,对戊唑·丙森锌最敏感,EC50值为82.99μg/mL;对辛菌胺醋酸盐和苯醚甲环唑次之,EC50值分别为139.51μg/mL和108.22μg/mL;而对甲基硫菌灵的敏感性最低,EC50值为267.55μg/mL。质量浓度为50μg/mL时,戊唑·丙森锌、苯醚甲环唑、辛菌胺醋酸盐和甲基硫菌灵对该菌株的抑制率分别为46.09%、43.48%、39.13%、27.83%。放线菌ZZ-9菌株在35℃下生长最好,15℃和45℃下生长受到明显抑制。

The mycelial growth rate method was used to determine the sensitivity of actinomycetes ZZ-9 strain to different temperatures and different agents,aiming to provide an important theoretical basis for the later biological preparation research.The results of the drug test showed that actinomycetes ZZ-9 showed some sensitivity to the four fungicides tested.Among them,actinomycetes ZZ-9 was the most sensitive to ethiazole and propionate zinc,with an EC50 value of 82.99μg/mL;ciprofloxacin acetate and difenoconazole followed by EC50 values of 139.51μg/mL and 108.22μg/mL;and the minimum EC50 value for thiophanate-methyl is 267.55μg/mL.The difenoconazole,ciprofloxacin acetate and thiophanate-methyl were 46.09%,43.48%,39.13% and 27.83%,respectively,when the concentration of thiophanate-methyl was 50μg/mL.The temperature test showed that the strain EZ-9 had the best growth at 35℃.Growth was significantly inhibited at 15℃ and 45℃.