质谱电喷雾离子源中电化学与电晕放电氧化还原反应的研究进展

Research Progress on Electrochemistry-induced and Corona Discharge-induced Redox Reactions in Electrospray Ionization Mass Spectrometry

质谱电喷雾离子化过程中包含两类氧化还原反应:电化学氧化还原和电晕放电氧化还原。一方面,这两类反应干扰谱图解析、降低分析物的检测灵敏度;另一方面,利用氧化还原的特性可发展新型离子源,提高电喷雾离子化过程中难离子化化合物的离子化效率,研究蛋白质相互作用等。该文系统地介绍了国内外对于电化学氧化还原反应和电晕放电氧化还原反应的最近研究进展,主要包括此两类反应的弊端、应用价值,以及控制两类反应的方法。最后总结了区分两种反应的方法,并对电喷雾离子源的发展进行了展望。

There are two kinds of redox reactions occurring in electrospray ionization mass spectrometry,i.e.electrochemistry and corona discharge-induced redox reactions.On one hand,the two kinds of redox reactions will complicate the mass spectra,and decrease the sensitivity for analyte detection.On the other hand,the reactions could be used to develop new ionization sources,improve the ionization efficiency for hardly ionized species and study the protein interaction.The research progress of electrochemistry and corona discharge-induced redox reactions at home and abroad was introduced in this review,including their advantages and disadvantages,as well as the methods used to control the two redox reactions.Finally,the methods used to distinguish the two kinds of oxidation reaction were summarized.