利用SSR标记鉴定杂交旱稻新品种鑫两优212纯度的研究

Application of SSR Molecular Marker Technology in Seed Purity Identification of New Hybrid Upland Rice Xinliangyou 212

杂交水稻纯度鉴定对水稻种子质量控制和降低生产经营风险具有重要意义。利用SSR标记技术,以籼稻杂交旱稻新品种鑫两优212及其亲本DNA为材料,从48对SSR引物中筛选出3对在杂交种鑫两优212中呈现双亲带型的引物。研究表明,这3对引物均可用于杂交种鑫两优212种子纯度鉴定。

It is important to identify the purity of the hybrid rice seed on managing rice seed quality and controlling risks.In this study,genomic DNA extracted from F1hybird of Xinliangyou212and its parental lines was used to SSR analysis with48pairs of primer,3pairs of SSR primers were selected,which with the male and the female parents polymorphism.The3pairs of SSR primers could be used to identify the purity of this hybrid.