HPLC法同时测定云南小粒咖啡中4种成分的含量

Determination of Four Active Components in Arabica of Yunnan by HPLC Method

[目的]建立云南小粒咖啡中绿原酸、葫芦巴碱、咖啡酸、D-(-)-奎宁酸含量的测定方法。[方法]采用Venusil ASB C18为色谱柱,甲醇-0.1%磷酸水为流动相,等度洗脱,流速0.9 m L/min,柱温35℃,检测波长:绿原酸和咖啡酸330 nm,葫芦巴碱和D-(-)-奎宁酸210 nm。[结果]绿原酸、葫芦巴碱、D-(-)-奎宁酸、咖啡酸分别在14.6～146.0μg/m L(r=1.000 0)、10.2～102.0μg/m L(r=1.000 0)、11.6～116.0μg/m L(r=0.999 8)、0.499 5～4.995 0μg/m L(r=0.999 8)的浓度范围内与峰面积线性关系良好,加标回收率为93.28%～97.46%。[结论]该方法简便、准确、重复性好,可用于云南小粒咖啡中绿原酸、葫芦巴碱、咖啡酸和D-(-)-奎宁酸含量的测定。

[Objective]To establish a method for the determination of chlorogenic acid,trigonelline,caffeic acid and D-(-)-quinic acid in Arabica of Yunnan by HPLC.[Methods]The HPLC conditions were ascertained as follows:Venusil ASB C18chmatgraphic column,mobile phase methanol plus0.1%phosphoric acid,isometric elution;flow rate0.9mL/min;column temperature35℃;determination wavelength:chlorogenic acid and caffeic acid with330nm,trigonelline and D-(-)-quinic acid with210nm.[Results]There were linear relationships between the peak area and contents of determination of chlorogenic acid,trigonelline,caffeic acid and D-(-)-quinic acid in the ranges of4.6~146.0μg/mL(r=1.0000),10.2~102.0μg/mL(r=1.0000),11.6~116.0μg/mL(r=0.9998),0.4995~4.9950μg/mL(r=0.999),respectively.The extraction recoveries varied between93.28%-97.46%.[Conclusion]The method was simple,accurate,reliable,and can be used to detect chlorogenic acid,trigonelline,caffeic acid and D-(-)-quinic acid in coffea arabica.