银纳米粒子比色传感器法测定食品及水样中双酚A

Determination of BPA in Food and Water by Silver Nanoparticles Based Colorimetric Sensor

[目的]研究一种基于银纳米粒子聚集使体系颜色发生改变的原理,建立新的生物传感器检测食品中的双酚A。[方法]采用柠檬酸还原法合成银纳米粒子并修饰核酸适配体。双酚A与核酸适配体结合发生变构效应导致银纳米粒子聚集,其特征峰以及颜色发生变化,研究体系特定波长吸光度比值与双酚A浓度的变化关系。[结果]在最佳优化条件下,该比色法在质量浓度为0.50～50.00 ng/m L呈现良好的线性关系,检出限为0.3 ng/m L。将该检测体系应用于BPA类似物的检测,特异性较好。[结论]该方法对食品中双酚A的检测有一定的应用前景。

[Objective]A new biosensor for the detection of bisphenol A(BPA)in food was developed based on the principle of color change caused by agglomeration of silver nanoparticles.[Method]Silver nanoparticles were synthesized by citric acid reduction method and modified with nucleic acid aptamers.The conjunctions of bisphenol A and aptamer lead to the agglomeration of silver nanoparticles,the characteristic peak and color change.The relationship between the ratio of absorbance of specific wavelength and the concentration of bisphenol A was studied.[Result]Under the optimum conditions,the colorimetric method showed a good linear relationship in the mass concentration range of0.50-50.00ng/mL,and the detection limit was0.3ng/mL.The system was applied to the detection of BPA analogues with good specificity.[Conclusion]This method can be applied to the determination of bisphenol A in food.