幼龄与成年沙鼠短暂性脑缺血再灌注后海马8-羟基脱氧鸟苷含量的比较

Comparison of hippocampus 8-hydroxydeoxyguanosine level between young and adult gerbils with transient cerebral ischemia reperfusio

目的比较幼龄与成年沙鼠短暂性脑缺血再灌注后脑组织8-羟基脱氧鸟苷(8-OHdG)的含量。方法采用短暂性双侧颈总动脉夹闭建立脑缺血再灌注模型。采用ELISA法检测脑卒中后8-OHdG含量,采用甲酚紫及F-JB染色法观察海马CA1区神经元的死亡情况,采用免疫组化染色法观察海马CA1及DG区8-OHdG的表达情况。结果缺血再灌注第4天,成年沙鼠海马CA1神经细胞明显死亡,而幼龄沙鼠几乎没有。缺血再灌注后,脑组织中8-OHdG含量逐渐增高,在6 h时达到最高峰,且成年沙鼠整体水平较幼龄沙鼠高。成年沙鼠CA1及DG区8-OHdG免疫活性变化与8-OHdG含量变化相似,幼龄沙鼠则无明显变化。结论脑缺血再灌注后,幼年沙鼠海马CA1和DG区相对低表达状态与其抗脑缺血有密切的联系。

Objective To compare the hippocampus 8-hydroxydeoxyguanosine(8-OHdG)level between young and adult gerbils with transient cerebral ischemia reperfusion.Methods The model of gerbils with transient cerebral ischemia reperfusion was established by occluding bilateral common carotid arteries.The 8-OHdG content after stroke was detected by ELISA,the death of neurons in hippocampal CA1 region was observed by cresol purple and F-JB staining,and the expressions of 8-OHdG in hippocampal CA1 and DG regions were observed by immunohistochemical staining.Results On the 4 th day after ischemia reperfusion,neurons in the hippocampal CA1 region of adult gerbils were apparently killed,but neurons death of young gerbils were hardly observed.After ischemia reperfusion,the level of 8-OHdG in the cerebral tissues increased gradually and reached the peak at 6 h,and the average level of 8-OHdG in adult gerbil was higher than that in young gerbils.In addition,the change of 8-OHdG immunocompetence in the hippocampal CA1 and DG regions in adult gerbils were similar with the change of 8-OHdG level,but the same change in young gerbils were not observed.Conclusion After ischemia reperfusion,the relatively low expressions of 8-OHdG in the hippocampal CA1 and DG regions in young gerbils are closely related to its ability to resist cerebral ischemia.