食管癌组织中基质金属蛋白酶-9水平的表达及临床意义

Expression and clinical significance of matrix metalloproteinase-9 in esophageal carcinoma tissues

目的探讨食管癌组织中基质金属蛋白酶-9(matrix metalloproteinase,MMP-9)水平的表达及临床意义。方法采用免疫组化法检测MMP-9在食管癌及癌旁正常食管组织中的表达。Image-pro plus图像分析软件对其表达强度进行定量分析,并用表达的阳性单位(positive unit,PU)反映其表达强度。结果研究组食管癌组织MMP-9水平高于正常食管组织对照组(P <0.001);不同性别、年龄、病理类型、病变位置食管癌患者MMP-9水平间差异无统计学意义(P>0.05),区域淋巴结转移及Ⅲ、Ⅳ期食管癌患者MMP-9水平分别高于无淋巴结转移及Ⅰ、Ⅱ期食管癌患者(P <0.05)。结论食管癌组织中MMP-9水平较正常组织异常增高,且随淋巴结转移及临床分期增加,其增高幅度更显著,但与患者年龄、性别及病理类型无关,临床可通过检测食管癌患者组织中MMP-9水平判断其病情进展状况。

Objective To explore the expressions and clinical significance of matrix metalloproteinase-9(MMP-9)in esophageal carcinoma tissues. Methods Immunohistochemistry was used to detect the expression of MMP-9 in esophageal carcinoma tissues and adjacent normal esophageal tissues.Image-pro plus image analysis software was used to given quantitative analysis on its expression intensity,and positive unit(PU)of expression was used to reflect its expression intensity. Results The expression of MMP-9 in esophageal carcinoma tissues(research group)was higher than that in normal esophageal tissues(control group)(P <0.001).There was no significant difference in the MMP-9 expression in patients with esophageal carcinoma of different genders,ages,pathological types and lesions sites(P >0.05),and the MMP-9 expression in patients with regional lymph node metastasis and stage Ⅲ、Ⅳ esophageal carcinoma was higher than that in patients with no lymph node metastasis and with stage Ⅰ、Ⅱ esophageal carcinoma(P <0.05). Conclusion The MMP-9 expression in esophageal carcinoma tissues is abnormally increased compared with that in normal tissues,and its increase is more significant with the increase of lymph node metastasis and clinical stages,but it has no significant correlation with the age,gender and pathological types of patients.The detection of MMP-9 expression in esophageal carcinoma tissues can judge the disease progression in clinical practice.