拟南芥突变体jar1的鉴定及其MeJA感受性分析

Identification and MeJA Sensitivity Analysis of Arabidopsis Mutant jar1

为了研究JAR1 (At2g46370)基因在茉莉酸调控机制中的作用,从拟南芥生物资源中心获得JAR1基因的T-DNA插入突变体jar1 (SALK_030821),对突变体中T-DNA插入位点和突变纯合性进行检测,筛选出纯合的jar1突变体。通过qRT-PCR检测jar1突变体中JAR1基因的表达水平,并进行茉莉酸敏感性实验,观察jar1幼苗在含有茉莉酸甲酯的培养基的生长情况。结果表明:纯合突变体中JAR1基因的表达量明显下降,仅为野生型植株的41. 2%; jar1幼苗对茉莉酸甲酯的敏感性显著下降。

1n order to study the role of JAR1(At2g46370)gene in the mechanism of JA signaling,a mutant jar1(SALK_030821)inserted by T-DNA was obtained from the Arabidopsis Biological Resource Center.The homozygous jar1 mutants were screened out after the position of T-DNA insertion were identified.The expression level of the JAR1 gene in jar1 mutants was analyzed by quantitative real-time qRT-PCR,JA sensitivity was detected,and the growth of the seedling in the medium containing MeJA was observed.The results show that the expression of JAR1 gene was dramatic reduction,it is about 41.2%of wild type plant.Comparing to the wild type,the sensibility of homozygous mutant jar1 seedling roots to MeJA was also decreased.