丛枝菌根真菌(AMF)对星油藤根腐病的抗性研究

Investigation on Resistance of Arbuscular Mycorrhizal Fungi(AMF) to Root Rot of Plukenetia volubilis

2015—2016年在云南省西双版纳傣族自治州随机调查不同发病程度地块及不发病地块150株星油藤的根系以及根系周围土壤,通过土壤湿筛法测定孢子密度,根系透明染色镜检法计算菌根侵染率与星油藤根腐病的发病率和发病程度,探明其根腐病的发生与丛枝菌根真菌多样性的关系,星油藤根际丛枝菌根真菌根系侵染率及孢子密度对星油藤根腐病发病率和发病程度的影响。结果表明:丛枝菌根真菌对根系侵染率达到90%,星油藤长势良好,而侵染率低于13%时,星油藤长势衰弱;而丛枝菌根真菌孢子密度达到6时,星油藤可以健康生长,当孢子密度低于2时,星油藤发病率高,且发病严重。丛枝菌根真菌可以增强星油藤的抗根腐病能力,并能显著降低星油藤根腐病的发病率。

From 2015 to 2016,the root and root soil of 150 Plukenetia volubilis in different morbidity and nonmorbidity plots were investigated randomly in Xishuangbanna Dai Autonomous Prefecture of Yunnan Province.Spore density was determined by wet sieve from the soil.The infection rate,incidence and degree of root rot was calculated by transparent staining microscopy.The relationship between root rot and the diversity of arbuscular mycorrhizal fungi,and the effects of root infection rate and spore density on the incidence and degree of root rot of P.volubilis were studied.The findings indicated that arbuscular mycorrhizal fungi on root infection rate of 90%,vines grew well,and the infection rate is lower than 13%,the vines growing weak.And arbuscular mycorrhizal fungi spore density reached 6,P.volubilis can healthy growth.When the spore density is lower than 2,P.volubilis incidence of a disease is very serious.Arbuscular mycorrhizal fungi can enhance the resistance of P.volubilis root rot and significantly reduce the incidence of this disease.