31个生物体FAD3蛋白的结构和系统进化

Structure and Phylogenetic Analysis of FAD3 Proteins from 31 Organisms

以31个生物体的35个FAD3全长蛋白为研究对象,利用生物信息学软件对其理化性质、保守结构域和系统进化等进行综合比较分析。结果表明:蛋白质平均含有393±22个氨基酸残基,相对分子质量为45 047±2 259,理论等电点为8.09±0.78,不稳定系数为33.31±4.33,亲水性指数为-0.15±0.06;多重序列比对结果显示,除外类群(Ct FAD3-HM214545.1)外,34个FAD3蛋白的相似性达71.9%,还存在PPF、HHLFPQ、YLRGGLTT、VLGHDCGHGSFS等多个保守的氨基酸富集区;系统进化树分析表明,单子叶植物与双子叶植物各聚一支,后汇聚在同一进化树中。

With 35 full-length of FAD3 proteins from 31 species,their physicochemical property,conserved structural domain and phylogenetic analysis were comprehensively compared and analyzed by using bioinformatics softwares. The results showed that,on average,their proteins contains 393± 22 amino acid residues,the relative molecular weight is 45 047 ±2 259,the theoretical isoelectric point is 8.09±0.78,the instability index is 33.31±4.33,and the grand average of hydropathicity is-0.15±0.06. The multiple sequence alignment showed that the comparability of 34 FAD3 proteins is 71.9 %besides the outgroup( Ct FAD3-HM214545.1),and there are multiple conservative amino acid rich motifs,such as PPF,HHLFPQ,YLRGGLTT and VLGHDCGHGSFS. By the phylogenetic analysis,the monocotyledon and dicotyledon have one branch each,and then converge in the same evolutionary tree.