脊髓损伤模型大鼠损伤节段的选择:网状Meta分析

Selection of injury segments in a rat model of spinal cord injury: network meta-analysis

背景:目前Allen’s打击法已经发展成为一种安全性和有效性相对较好的造模方式,脊髓损伤节段的选择也是建立理想的脊髓损伤模型非常重要的因素。目的:运用网状Meta分析的方法,比较对SD大鼠胸T_8,T_9,T_(10),T_(11),T_(12)造成脊髓损伤有效性的异同,为脊髓损伤大鼠模型损伤节段的选择提供有力证据。方法:计算机检索PubMed、中国知网(cnki)、重庆维普(VIP网)、万方数据库。英文检索主题词:"spinal cord injuries","rats","Models,Animal",中文检索主题词:"脊髓损伤模型","大鼠","BBB评分"。检索国内外所有截止时间2018年6月5日公开发表的所有文献。入选的文献均为Allen’s打击法建立脊髓损伤大鼠模型,损伤量以25 g·cm为标准,以仅进行椎板切除的大鼠为假手术组,采用Basso Beattie Bresnahan(BBB)下肢运动功能评分为观察3,7,14,28 d时间点评分。网状Meta分析比较T_8,T_9,T_(10),T_(11),T_(12)不同节段脊髓损伤有效性的排序。结果与结论:(1)最终纳入研究19篇,共703只大鼠纳入研究;(2)在3,7,14,28 d时间点不同损伤节段脊髓损伤后的BBB评分较假手术组明显下降(P≤0.05);(3)T_(10)节段脊髓损伤对下肢功能BBB评分影响最大、有效性最高;(4)不同损伤节段脊髓损伤的有效性最终的排序是:T_(10)损伤> T_9损伤> T_(11)损伤> T_8损伤> T_(12)损伤>假手术。

BACKGROUND:Allen’s weight-drop method has become a safe and effective modeling method,and choosing the injury segments of the spinal cord is an important factor for establishing ideal animal models of spinal cord injury.OBJECTIVE:To compare the the similarities and differences in the effectiveness of spinal cord injury at T8,T9,T10,T11,and T12 in Sprague-Dawley rats by network meta-analysis,thus providing evidence for the selection of lesion segments in rat models of spinal cord injury.METHODS:PubMed,CNKI,VIP and WanFang databases were searched with the keywords of“spinal cord injuries,rats,models,animal,BBB scores”in English and Chinese,respectively for the literature published before June 5,2018.The inclusion criteria were as follows:the modeling method of spinal cord injury was Allen's weight-drop(25 g·cm)method,rats only undergoing laminectomy were included in sham operation group,and low extremity function was assessed by Basso Beattie Bresnahan scores at 3,7,14 and 28 days.The order of the effectiveness of spinal cord injury at T8,T9,T10,T11,and T12 was compared by network meta-analysis.RESULTS AND CONCLUSION:(1)Nineteen studies were included,involving 703 rats.(2)Basso Beattie Bresnahan scores in the spinal cord injury rats at different segments at 3,7,14 and 28 days were significantly decreased compared with the sham operation group(P≤0.05).(3)Spinal cord injury at T10 showed largest effect on Beattie Bresnahan scores.(4)The order of effectiveness of spinal cord injury at different segments was:T10>T9>T11>T8>T12>sham operation.