黑木耳多糖对TM_3细胞增殖的影响

Effects of auricularia auricula polysaccharide on the proliferation of TM_3 cells

目的 MTT法观察不同浓度黑木耳多糖及其含药血清对大鼠睾丸间质细胞(TM_3细胞)增殖的影响,为黑木耳多糖抑制大鼠生殖系统功能的机制研究提供理论依据。方法大鼠灌胃制备的黑木耳多糖溶液和黑木耳多糖含药血清分别作用于TM_3细胞,采用MTT法观察两种处理方式对TM_3细胞增殖的影响。结果不同浓度黑木耳多糖处理组与对照组之间的吸光度值差异极显著(P <0. 01),不同浓度黑木耳多糖组之间的吸光度值差异极显著(P <0. 01)或显著(P <0. 05),随着黑木耳多糖浓度增加,吸光度值先增加,后降低。不同浓度黑木耳多糖含药血清组与对照血清组之间的吸光度值差异极显著(P <0. 01)或显著(P <0. 05),各浓度黑木耳多糖含药血清组吸光度值均明显低于对照血清组。结论黑木耳多糖溶液对TM_3增殖表现为低浓度促进,高浓度抑制,黑木耳多糖含药血清对TM_3细胞增殖表现为明显抑制作用。

Objective To observe the effect of different concentration of auricularia auricula polysaccharide(AAP)on the proliferation of TM3 cell,and provide a theoretical basis for AAP inhibiting the reproductive system function in rats.Methods AAP in the serum and AAP solution were employed to treat TM 3.The effects of two preparations on TM3 proliferation were compared by MTT.Results The difference between the AAP group and the control group was significant(P<0.01).The difference between different concentrations of AAP groups was significant(P<0.01 P<0.05).With the increase of AAP concentration,the absorbance value increased first and then decreased.The difference between the serum groups with different concentrations of AAP and the control group was significant(P<0.01 or P<0.05),and the absorbance value of AAP serum group was significantly lower than that in the control group.Conclusion AAP could promote cells proliferation at low concentration while inhibite TM3 proliferation at high concentration.AAP in the serum show a significant inhibitory effect on the proliferation of Leydig cells.