Sox5促进胰腺癌细胞迁移侵袭和上皮间质化

Sox5 Promotes Migration,Invasion and Epithelial Mesanchymal Transition of Pancreatic Cancer Cells

目的:探讨Sox5对胰腺癌细胞迁移侵袭和上皮间质化的影响。方法:人正常胰腺细胞HPDE6-C7(HPDE6-C7组)、胰腺癌细胞PANC-1(PANC-1组)和AsPC-1(AsPC-1组)正常培养至对数生长期,采用qRT-PCR法和Western blot法分别检测各组细胞中Sox5mRNA和蛋白水平。将shSox5、shControl、pcDNA 3.1-Sox5和载体pcDNA 3.1以脂质体法转染胰腺癌细胞PANC-1,分别标记为shSox5组、shControl组、pcDNA 3.1-Sox5组和Scrambled组,采用qRT-PCR法检测各组细胞中Sox5、E-cadherin、N-cadherin、Vimetin、Snail和Twist1的mRNA表达;Western blot检测各组细胞中Sox5、E-cadherin、N-cadherin、Vimetin、Snail和Twist1的蛋白表达;Transwell法检测各组细胞的迁移和侵袭。结果:与HPDE6-C7组相比,PANC-1组和AsPC-1组中Sox5mRNA和蛋白水平均升高(P<0.01);与shControl组比较,shSox5组Sox5、N-cadherin、Vimetin、Snail和Twist1的mRNA和蛋白以及细胞迁移和侵袭水平均显著降低(P<0.01),E-cadherin mRNA和蛋白水平均显著升高(P<0.01);与Scrambled组比较,pcDNA-3.1-Sox5组Sox5、N-cadherin、Vimetin、Snail和Twist1的mRNA和蛋白以及细胞迁移和侵袭水平均显著升高(P<0.01),E-cadherin mRNA和蛋白水平均显著降低(P<0.01)。结论:Sox5可促进胰腺癌细胞迁移、侵袭和EMT,将为胰腺癌的靶点治疗提供新靶标。

Objective:To investigate the effects of Sox5 on migration,invasion and epithelialization of pancreatic cancer cells.Method:Human normal pancreatic cells HPDE6-C7(HPDE6-C7 group),pancreatic cancer cells PANC-1(PANC-1 group)and AsPC-1(AsPC-1 group)were cultured normally to logarithmic growth phase.The levels of Sox5 mRNA and protein in each group were detected by qRT-PCR and Western.shSox5,shControl,pcDNA 3.1-Sox5 and vector pcDNA 3.1 were transfected by liposome method.Pancreatic cancer cells PANC-1 were stained with sh Sox5 group,shControl group,pcDNA 3.1-Sox5 group and Scrambled group,respectively.qRTPCR was used to detect the mRNA expression of Sox5,E-cadherin,N-cadherin,Vimetin,Snail and Twist1 in each group.The expressions of Sox5,E-cadherin,N-cadherin,Vimetin,Snail and Twist1 were detected by Western blot.The migration and invasion of cells in each group were detected by Transwell method.Results:Sox5 mRNA and protein levels were highly increased in PANC-1 group and AsPC-1 group compared with HPDE6-C7 group(P<0.01).compared shSox5 group with shControl group,the mRNA and protein levels of Sox5,N-cadherin,Vimetin,Snail,Twist1,migration and imasion were significantly decreased(P<0.01),E-cadherin mRNA and protein levels were significantly increased(P<0.01).Wascompared pcDNA-3.1-Sox5 group with Scrambled group,the mRNA and protein levels of Sox5,N-cadherin,Vimetin,Snail,Twist1,migration and imasion were significantly increased(P<0.01),and E-cadherin mRNA and protein levels were significantly decreased(P<0.01).Conclusion:Sox5 could promote pancreatic cancer cell migration,invasion and epithelial-mesenchymal transitions,and will provide new targets for the treatment of pancreatic cancer.