不同碘浓度对大鼠甲状腺摄碘功能的影响

Effects of Different Iodine Concentrations on Thyroid Iodine Uptake in Rats

目的观察不同碘摄入量对大鼠促甲状腺激素受体(TSHR)mRNA、钠碘转运体(NIS)m RNA以及血清过氧化物酶抗体(TPOAb)表达水平的影响。方法 SD大鼠30只,随机分成低碘组(LI)、适碘组(NI)和高碘组(HI),每组10只。于实验第8、16周分批处死。实时荧光定量聚合酶链式反应法测定TSHR mRNA及NIS mRNA表达水平。酶联免疫分析法观察血清TPOAb浓度。结果实验8周,低碘组和高碘组TSHR mRNA及NIS mRNA表达水平均低于适碘组,差异有统计学意义(P<0.05);且血清TPOAb浓度高于适碘组,但差异无统计学差异(P>0.05);实验16周,高碘组和低碘组的TSHR mRNA和NISmRNA表达水平均明显低于适碘组,差异有统计学意义(P<0.05);血清TPOAb浓度高于适碘组,差异有统计学意义(P<0.05)。结论包括碘过量和碘缺乏在内的长期碘营养障碍均可降低NIS及TSHR的正常表达,同时提高甲状腺自身抗体的浓度,影响甲状腺摄碘功能。

Objective To investigate the effects of different iodine intake on the expression of(TSHR)mRNA,sodium iodide transporter(NIS)mRNA and peroxidase antibody(TPOAb)in rat thyroid stimulating hormone receptor.Methods30SD rats were randomly divided into two groups:low iodine group(LI),normal iodine group(NI)and high iodine group(HI),)10rats in each group.The rats were executed in batches at the8th,16th week.The expression levels of TSHR mRNA and NIS mRNA were measured by real-time fluorescence quantitative polymerase chain reaction.The concentration of serum TPOAb was observed by enzyme linked immunosorbent assay.Results At the8th week of the experiment,the expression of TSHR mRNA and NIS mRNA in iodine deficiency group and high iodine group were lower than that in normal iodine group,the difference was statistically significant(P<0.05).The concentration of serum TPOAb was higher than that of iodine group,but there was no significant difference(P>0.05).At the16th week of experiment,the expression levels of TSHR mRNA and NISmRNA in high iodine group and low iodine group were significantly lower than those in normal iodine group,the difference was statistically significant(P<0.05).The concentration of serum TPOAb was higher than that of iodine group,the difference was statistically significant(P<0.05).Conclusion Chronic iodine nutritional disorder,including iodine excess and iodine deficiency,can decrease the normal expression of NIS and TSHR,increase the concentration of thyroid autoantibodies and affect the iodine uptake function of thyroid.