FBXW7在糖尿病心肌病中的表达

Expression of FBXW7 in diabetic cardiomyopathy

目的:检测含F框/WD重复域蛋白7(FBXW7)在1型糖尿病大鼠心肌中的表达,探究其在糖尿病心肌病发生发展中的作用。方法:实验将大鼠分为非糖尿病组(ND组)及糖尿病4、8和12周模型组(DM组),采用链脲佐菌素(60 mg/kg)一次性腹腔注射建立1型糖尿病大鼠模型。HE染色法观察心肌病理结构变化,Western blot和免疫组化法检测心肌中FBXW7蛋白的表达变化。结果:显微镜下可见糖尿病组大鼠心肌局灶性心肌细胞变性、坏死。Western blot和免疫组化结果均显示,4周、8周及12周DM组大鼠心肌中的FBXW7表达相对于ND组显著增加(P <0. 01),但是相比于4周和8周DM组,12周DM组大鼠心肌中的FBXW7表达相对下降(P <0. 05)。结论:随着糖尿病病程的延长,FBXW7在糖尿病心肌病大鼠心肌组织中的表达呈现先升高后降低的趋势,提示其在糖尿病心肌病发生发展中发挥了一定的作用。

AIM:To investigate the expression of F-box/WD repeat-containing protein7(FBXW7)in the myocardium of type1diabetic rats and to clarify its role in the development of diabetic cardiomyopathy.METHODS:All rats were randomly divided into non-diabetic(ND)group,4-week diabetes mellitus(DM)model group,8-week DM mo-del group and12-week DM model group.The DM model was prepared by intraperitoneal injection of streptozotocin(60mg/kg).The change of myocardial pathological structure was investigated by HE staining.The FBXW7expression level in the myocardium was determined by Western blot and immunohistochemistry methods.RESULTS:DM induced cardiomyocyte degeneration and necrosis as shown by cardiac histological analysis.Both Western blot and immunohistochemistry results showed that the expression level of FBXW7was significantly increased in4-week,8-week and12-week DM groups compared with control group(P<0.01).However,The FBXW7expression level in12-week DM group was decreased compared with4-week and8-week DM groups.CONCLUSION:With the development of diabetes,the expression of FBXW7in the myocardium of the rats with diabetic cardiomyopathy shows a tendency to increase first and then decrease,suggesting that it plays some roles in the development of diabetic cardiomyopathy.