黄芪多糖提取工艺优化及其抗氧化活性研究

Study on Optimization of Extraction Process of Astragalus Polysaccharides and Its Antioxidant Activity

目的采用微波辅助法对黄芪多糖的提取工艺及其抗氧化活性进行研究。方法根据单因素试验结果,采用Box-Behnken试验设计和响应面分析法,探讨液料比、提取时间和提取功率对黄芪多糖提取率的影响,考察最佳提取工艺。采用二苯基三硝基苯肼(DPPH·)法和羟基自由基(OH·)法研究其抗氧化活性。结果黄芪多糖的最佳提取工艺为液料比30. 46 m L/g,提取时间2. 21 h,提取功率204. 45 W;在此条件下,多糖提取率为9. 69%;黄芪多糖对DPPH·和OH·有一定的清除能力。结论采用响应面分析法对黄芪多糖提取条件进行优化合理可行;黄芪多糖有一定的抗氧化能力。

Objective To study the extraction process and antioxidant activity of astragalus polysaccharides by microwave-assisted method. Methods Based on the single factor test results,Box-Behnken test design and response surface methodology were used to investigate the effects of liquid-to-material ratio,extraction time and power on the extraction rate of astragalus polysaccharides,and the optimum extraction process was investigated. The antioxidant activity of astragalus polysaccharides was studied by DPPH · method and OH· method. Results The optimum extraction process of astragalus polysaccharides was as follows: the ratio of liquid to material was 30. 46 m L/g,the extraction time was 2. 21 h,and the extraction power was 204. 45 W. Under these conditions,the extraction rate of polysaccharides was 9. 69%. Astragalus polysaccharides had certain scavenging capacity for DPPH · and OH ·. Conclusion It is reasonable and feasible to optimize the extraction process of astragalus polysaccharides by response surface methodology method,and the astragalus polysaccharides have certain antioxidant capacity.