妊娠期糖尿病SD大鼠对子代糖脂代谢的影响

Impacts of SD rats with GDM on progeny glucose and lipid metabolism

目的探讨妊娠期糖尿病SD大鼠对子代糖脂代谢的影响。方法选择30只8周龄SD雌性大鼠,随机均分为观察组及对照组,分别与健康雄性大鼠交配,在妊娠第1 d:观察组单次腹腔注射40 mg/kg链脲佐菌素,构建妊娠期高血糖动物模型;对照组则腹腔注射同体积柠檬酸-柠檬酸钠缓冲液,两组子代分别记为子代观察组与子代对照组。在分娩后7 d及喂养20 w时,对比两组子代糖耐量实验(GTT)血糖值;在喂养至21 w时,检测两组子代血脂水平和肝脏相关基因表达量。结果GTT结果显示,在分娩后7 d时,两组子代血糖值比较无显著差异(P> 0.05),但喂养20 w时,子代观察组血糖明显高于子代对照组(P <0.05)。在21 w时,子代观察组的TC、TG和LDL-C水平显著高于子代对照组(P <0.05),而HDL-C明显低于子代对照组(P <0.05)。在21 w时,子代观察组肝脏PPARα、Ehhadh相对表达量明显高于子代对照组(P <0.05),miRNA-130 a、ASL3明显低于子代对照组(P <0.05),而两组CPT-1α、miRNA-214、miRNA-181a及miRNA-34a无显著差异(P> 0.05)。结论妊娠期糖尿病可引起子代成年后糖脂代谢紊乱,而肝脏相关基因表达异常可能是糖脂代谢紊乱的原因。

Objective To explore the impacts of SD rats with gestational diabetes mellitus(GDM)on progeny glucose and lipid metabolism.MeAods A total of 30 eight-week-old SD female rats were selected and randomly divided into an observation group and a control group.These female rats mated with healthy male rats respectively.On the first day of pregnancy:the animal model of hyperglycemia during pregnancy was established by single intraperitoneal injection of 40 mg/kg streptozotocin in the observation group;the control group was injected with the citric acid-sodium citrate buffer of the same volume by intraperitoneal injection.The progenies of the two groups were recorded as progeny observation group and progeny control group.Seven days after delivery and after feeding for 20 weeks,the blood glucose level in the progeny glucose tolerance test(GTT)were compared between the two groups;after feeding for 21 weeks,the progeny blood lipid level and the expression quantity of liver-related gene in the two groups were detected.Results The results of GTT showed that,seven days after delivery,there was no significant difference in the progeny blood glucose level between the two groups(P>0.05).However,after 20 weeks of feeding,the blood glucose in the progeny observation group was significantly higher than that in the progeny control group(P<0.05).In the 21st week,the levels of TC,TG and LDL-C in the progeny observation group were significantly higher than those in the progeny control group(P<0.05),but the levels of HDL-C were significantly lower than those in the progeny control group(P<0.05).In the 21st week,the relative expression quantity of liver PPARa and Ehhadh in progeny observation group was significantly higher than that in the progeny control group(P<0.05),but the miRNA-130 a and ASL3 were significantly lower than that in the progeny control group(户<0.05).There was no significant difference in the CPT-1a,miRNA-214,miRNA-181a and miRNA-34a between the two groups(P>0.05).Conclusion GDM can cause glucose and lipid metabolism disorders in progenies after being an adult.The abnormal expression of genes related to liver may be the cause of glucose and lipid metabolism disorders.