静电纺核-壳纳米包装膜的制备及其抗菌性能

Preparation and antibacterial properties of electrospun core-shell nanoscale packaging films

为解决抗菌包装中精油包埋的问题,采用乳液静电法制备了外壳为羧甲基壳聚糖(CMCS),内核为百里香精油的纳米纤维膜,研究了乳液的黏度、电导率以及粒径对纳米纤维的影响,借助扫描电子显微镜和透射电子显微镜观察了纤维膜的微观形貌,采用抑菌圈法测试了纤维膜的抑菌性能。结果表明:质量比为9∶1的羧甲基壳聚糖和聚氧化乙烯混合液为水相溶液,百里香精油为油相,吐温80为乳化剂,当三者体积比为36.7∶4∶1或22.8∶4∶1时,均可形成稳定的乳液结构;当纺丝液流量为0.5 mL/h、电压为16～24 kV、纺丝距离为11 cm时,百里香精油成功包裹在羧甲基壳聚糖纤维内,且随着电压升高,纤维黏连现象减弱;核-壳纳米纤维膜对金黄色葡萄球菌、大肠杆菌、灰霉、青霉的抑菌圈直径分别为18.2、11.0、32.5和29.2 mm,抑菌效果较好。

In order to solve the embedding of essential oil in antibacterial packaging,a nanofiber membrane with carboxymethyl chitosan(CMCS)as the shell and thyme essential oil as the core was prepared by an emulsion electrostatic process.The effects of viscosity,electrical conductivity and particle size of the electrospinning emulsion on nanofiber were studied.The microscopic morphology of the fiber membrane was observed by scanning electron microscopy and transmission electron microscopy.The bacteriostatic properties of the fiber membrane were tested by the inhibition zone method.The results show that carboxymethyl chitosan and poly(ethylene oxide)at a mass ratio of 9∶1 is used as the aqueous phase solution,thyme essential oil is used as the oil phase and Tween 80 is used as the emulsifier.When the volume ratio of the three materials is 36.7∶4∶1 or 22.8∶4∶1,a stable emulsion structure can be formed.Under the electrospinning conditions of spinning solution flow rate of 0.5 mL/h,voltage of 16-24 kV and spinning distance of 11 cm,thyme essential oil is successfully coated in carboxymethyl chitosan fiber,and the adhesion phenomenon decreases with the voltage increase.The inhibition zone diameter of the core-shell nanofiber membrane for Staphylococcus aureus,Escherichia coli,Botrytis cinerea and Penicillium is 18.2,11.0,32.5 and 29.2 mm respectively,and the bacteriostatic effect is good.