冷等离子体种子处理对铜胁迫下小麦种子萌发与幼苗生长的影响

The effects of cold plasma treatment on wheat seed germination and seedling growth under copper stress

为了评价冷等离子体种子处理对小麦种子萌发和生长抗重金属胁迫能力的影响,以4种不同功率的冷等离子体处理的3种小麦种子为研究对象,在3个不同浓度的硫酸铜胁迫下进行萌发成苗试验。通过测定种子的发芽率、根长、苗长以及根、苗中的Cu含量,计算发芽指数、种子活力指数,研究了冷等离子体种子处理对铜胁迫作用下小麦种子萌发与幼苗生长的影响。结果表明:冷等离子体种子处理对小麦在铜胁迫下的发芽率、发芽指数影响不显著,但提高了种子的萌发活力,根长和苗长最多分别提高了46.2%和145.4%,活力指数最多提高了82.1%。在低浓度Cu胁迫时,冷等离子体处理能够促进根、苗对Cu的吸收;而高浓度Cu胁迫时,冷等离子体在一定程度上能够提高Cu的转运能力,缓解了Cu对种子萌发活力、苗长和根长的抑制作用与毒害作用。研究表明,适宜功率的冷等离子体种子处理能够增强种子萌发和幼苗生长对重金属胁迫的抗性。

Several studies have reported that cold plasma treatment technology can not only enhance the resistance of crop seeds to salinity,drought,and cold,but can also improve the yield and quality of agricultural products.However,little information has been obtained on increasing the heavy metal resistance of crops germinated from seeds treated with cold plasma technology.To assess the effect of cold plasma treatment on the heavy metal resistance of seeds germinated,the germination and seedling growth of three varieties of wheat were investigated at three copper stress levels and four cold plasma power treatments.Seed germination rate,root length,sprout length,and copper concentration in the wheat seedlings were measured,and the germination and vigor indices,absorption coefficient,and transfer factors were calculated.The results showed that cold plasma treatments did not significantly affect the germination rates and indices under copper stress,but vigor index was improved markedly.The root length,sprout length,and vigor index increased by46.2%,145.4%,and82.1%,respectively,under the optimal cold plasma power treatment compared to those with no cold plasma treatments.At low copper concentrations,the amount of copper absorbed by the roots and sprouts increased under the cold plasma power treatment.However,at the high copper concentrations,cold plasma treatments improved the transport capacity of copper from roots to sprouts,which could alleviate the toxic effects of copper stresses on seed germination and root growth.These results suggest that cold plasma could effectively alleviate the toxic effects of copper on wheat.