摘要
铜离子在基因表达、维持蛋白质结构和功能等方面起到了重要作用,因此设计并合成高灵敏检测铜离子的多肽荧光探针对于准确了解铜离子的生理学作用至关重要。利用荧光分子"Off-On"模式,设计并合成了荧光探针RW。在乙腈-HEPES(10-2mol/L; pH=7.4;V/V=2∶8)缓冲溶液中实现了对铜离子的高灵敏高选择性荧光检测,并进行了探针RW荧光性质研究。
Copper ions play an important role in gene expression,protein structure and function maintainance.Therefore,the design and synthesis of fluorescent probes with high sensitivity for the detection of copper ions is of great importance in understanding the physiological function of copper ions.A novel probe RW is designed and synthesized by the fluorescent molecule“Off-On”mechanisation.The highly sensitive and selective fluorescence detection of copper ions is carried out in the system of acetonitrile-HEPES(10-2 mol/L;pH=7.4;V/V=2∶8)buffer solutions.The fluorescence properties of probe RW are also studied.
作者
王鹏
WANG Peng(College of Chemistry and Chemical Engineering,China West Normal University,Nanchong Sichuan 627009,China;Provincial Key Laboratory of Chemcial Synthesis and Pollution Control,China West Normal University,Nanchong Sichuan 627009,China)
出处
《西华师范大学学报（自然科学版）》
2018年第4期390-394,共5页
Journal of China West Normal University(Natural Sciences)
基金
国家自然科学基金项目(21471071)
西华师范大学博士启动基金项目(17E048)
关键词
罗丹明B
色氨酸
荧光探针
铜离子
Rhodamine B
tryptophan
fluorescence probe
copper ions
