苏氨酸废母液色谱分离实验与探讨

Recovery of threonine from waste mother liquor by chromatographic separation

把苏氨酸回收率及残糖去除情况作为指标,通过设置不同的实验环境对苏氨酸废母液进行回收,从而确定出最佳实验条件。研究结果表明:选用进口钙型(XA1)转氨型树脂,进料温度65℃,进料流量80～90mL/min,洗脱流量85～95mL/min,出料流量25～30mL/min,洗脱水pH在10左右,回料pH在9～10时,可以有效地将无机盐、糖与苏氨酸分开,把苏氨酸回收率提高到96.2%以上,降低生产损失。

The chromatographic separation conditions for threonine recovery from waste mother liquor was studied.The optimal conditions were summarized as follows:calcium ion-exchange resin(XA1),feeding temperature65℃,feeding flow rate80-90mL/min,mobile phase flow rate85-95mL/min,discharge flow rate25-30mL/min,eluant pH10,feed back pH9-10.Under these conditions,inorganic salt and sugar could be effectively separated from threonine,and the recovery of threonine was increased to above96.2%,reducing the production loss.