分枝杆菌病噬菌体33D基因组学特征及抗结核潜力

Genetic information of mycobacteriophage 33D and the potentials in control of tuberculosis

目的研究分枝杆菌噬菌体33D的基因组特性并探索其抗结核治疗的潜力。方法通过鸟枪法完成33D全基因组测序;使用DNAStar、TRF、Promoter predictions、tRNAscan-SE、Glimmer、BLAST软件分别对33D基因组进行分析。结果噬菌体33D基因组为线性双链DNA,全长50036bp;含有84个推定基因,17个已知功能的基因。其中噬菌体DNA复制相关基因:gene6、7、8、9和gene 14;裂解酶基因:gene 47和gene 48。结论噬菌体33D为烈性噬菌体,具有抗结核潜力,未发现致病基因,可作为"鸡尾酒疗法"的候选噬菌体。

Objective To explore the genetic information of phage 33D and its anti-tuberculosis potentials.Methods The genome of phage 33D were sequenced.The gene annotations of those genomes were completed by DNAStar,TRF,Promoter predictions,tRNAscan-SE,Glimmer and BLAST.Results The genome of phage 33D was a dsDNA sequence of 50036 bp.The genome of phage 33D encodes 84 gene,17 gene functions had been identified by BLAST comparison.We propose that gene47 and gene 48 could encode lyases.We proposed that gene6,7,8,9 and 14 were related to DNA Replication.Conclusion 33D is lytic phage which have potentials in control of tuberculosis and have not virulence gene.Phage 33D could be used in phage cocktail therapy.