胶体金免疫层析方法检测肺炎链球菌细胞壁多糖抗原

Gold Immunochromatography Assay for Streptococcus pneumoniae C-polysaccharide

建立一种简易快速、特异性高的胶体金免疫层析法(GICA)用于检测肺炎链球菌细胞壁多糖抗原(C-Ps).根据双抗体夹心原理,将C-Ps单克隆抗体标记于胶体金颗粒,建立检测方法.实验结果表明该试纸条操作简便,肉眼于15,min内即可判定结果.试纸条对肺炎链球菌具有高度特异性,与百日咳鲍特菌、脑膜炎奈瑟氏菌等其他重要呼吸道致病菌无交叉反应.室温保存12个月,其检测结果无明显变化.与现行检测方法相比较,该研究制备的试纸条具有操作简便、检测快速、检出限低、特异性强、稳定性好等优点,其灵敏度和特异性分别为70%,和100%.而且与国外同类产品(美国BinaxNOW?产品)相比,灵敏度具有较高的一致性,而且特异性可达到100%,,性价比更高,为肺炎链球菌感染疾病的临床检测与早期诊断提供了一个新的手段.

A simple,rapid and specific gold immunochromatography assay(GICA)was developed for the detection of Streptococcus pneumoniae C-polysaccharide(C-Ps).Based on the double-antibody sandwich principle,monoclonal antibody against C-Ps labeled with colloidal gold was used as the detection reagent.Our data demonstrated that the strip could be easily operated and the results could be rapidly judged within 15,min by naked eyes.The assay is highly specific to Streptococcus pneumoniae and showed no cross-reaction to Bordetella pertussis,Neisseria meningitides and other important respiratory pathogens.There was no significant change in sensitivity and specificity when the GICA kit was stored at room temperature for 12,months.Compared with the current detection methods,the new method has the advantages of simple operation,rapid detection,low detection limit,high specificity,and good stability.The strip had a sensitivity of 70%,and a specificity of 100%,.The performance of the strip was highly comparable with similar products in sensitivity and specificity such as BinaxNOW?,which was developed by a US company.In conclusion,the GICA-based kit described in this paper could potentially be used for clinical detection and early diagnosis of Streptococcus pneumoniae infection.