摘要
采用超声波辅助碱法提取栝楼叶蛋白.在单因素实验的基础上,通过正交实验得到了超声波辅助碱法提取栝楼叶蛋白的最佳工艺条件.在温度为55℃、pH值为10、超声时间为40min、超声功率为150 W、料液比为1∶25(g∶mL)、NaCl的浓度为0.5mol/L的最佳工艺条件下,叶蛋白提取率达到23.26%.
Protein was extracted from leaves of trichosantheskirilowii Maxim by alkali extraction method.Ultrasonic was used to increase the protein extraction rate and decrease the extraction time.Through single-factor tests and orthogonal experimental method,the optimal conditions for ultrasonic-assisted alkali extraction were determined as follows:temperature55℃,pH10,ultrasonic time40min,the ultrasonic power150W,the ratio of material to liquid l:25(g/mL)and the concentration of NaCl0.5mol/L.With these technical conditions,protein yield was about23.26%.
作者
刘爱良
段绍雄
熊利芝
LIU Ailiang;DUAN Shaoxiong;XIONG Lizhi(Yiyang No. 16 Middle School,Yiyang 413000,Hunan China;Yiyang No. 1 Middle School,Yiyang 413000,HunanChina;College of Biological Resources and Environmental Sciences,Jishou University,Jishou 416000,Hunan China)
出处
《吉首大学学报（自然科学版）》
CAS
2018年第6期56-60,共5页
Journal of Jishou University(Natural Sciences Edition)
基金
国家自然科学基金资助项目(51364009)
吉首大学校级项目(JGY201641
JDY16013
JDX16016
JDX16017)
吉首大学研究生培养创新基地项目([2015]20号)
林产化工工程湖南省重点实验室开放基金项目(JDZ201503)
生态旅游湖南省重点实验室开放项目(JDSTLY1612)
杜仲综合利用技术国家地方联合工程实验室(NLE201603)
矿物清洁生产与绿色功能材料开发湖南省重点实验室开放项目(MCPEGFM2006010)
关键词
栝楼
叶蛋白
超声波辅助
碱提取
Trichosanthes kirilowii Maxim
leaves protein
ultrasonic -assisted alkali extraction
