红锥茎段外植体消毒和初代培养

Research on Explant Disinfection and Primary Culture of Castanopsis hystrix Stem

红锥(Castanopsis hystrix)是华南地区重要的优质乡土阔叶树种,研究以红锥优树茎段为材料,研究不同消毒剂及处理时间、茎段木质化程度、留叶方式、光照强度和培养基对茎段初代培养试验的影响。结果表明,以红锥半木质化优树茎段为外植体,75%酒精5 s,0.1%升汞5 min,保留1/2叶,接种于改良MS+1.0 mg/L 6-BA+0.2 mg/L KT+0.2 mg/L NAA培养基,置于3 000 Lux光照强度下培养诱导效果最佳。

Castanopsis hystrix is an important high quality native broad-leaved tree species in Southern China.This paper studied the influences of different disinfectants and treating time,maturity level,way of keeping leaves,light intensity and culture medium on primary culture of C.hystrix stem.The stems were employed as initial explants,origins of which were superior individuals.The results showed the best induction rate when the stems were treated with 75%alcohol for 5 s,0.1%mercuric chloride for 5 min,kept 1/2 leaf,vaccinated in culture medium:MS+1.0 mg/L 6-BA+0.2 mg/L KT+0.2 mg/L NAA under 3 000 Lux light intensity.