CRISPR/Cas9基因编辑技术及其在动物细胞系构建中的应用

Application of CRISPR/Cas9 Mediated Genome Editing in Construction of Animal Cell Lines

CRISPR/Cas9[Clustered regular interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9]系统是广泛存在于细菌和古生菌中可降解入侵病毒和噬菌体DNA的一种基因定点编辑技术,由于其具有快速、精准、高效,易于设计,且特异性高等优势,得到了广泛的应用。概述了CRISPR/Cas9基因编辑技术的发展历程,并介绍其结构和编辑原理、在动物细胞系构建中的应用及展望,以便为更合理地应用和改进该技术提供系统的文献参考。

CRISPR/Cas9[Clustered regular interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9]system is a gene fixed-point editing technology widely found in bacteria and archaea that degrades invasive virus and bacterio phage DNA.It has been widely used because of its rapid,accurate,efficient,easy to design,and high specificity.The development of CRISPR/Cas9gene editing technology was reviewed,its structure and editing principle,its application and prospect in animal cell system construction were introduced in order to provide systematic reference for more rational application and improvement of the technology.