赤霞珠葡萄皮渣酚类物质提取及抗氧化性研究

Study on Extraction and Antioxidation of Phenolic Compounds from Cabernet Sauvignon Grape Pomace

研究赤霞珠葡萄皮渣中酚类物质提取和抗氧化性,为其资源化开发利用奠定基础。以总酚提取率为评价指标,采用单因素和正交试验优化提取条件。以VC为参照测定提取液对ABTS+自由基、DPPH自由基、羟自由基清除力和铁离子还原力。结果显示:相同条件下,赤霞珠葡萄籽中单宁和酚类物质提取率高于葡萄皮,最适条件为:料液比1∶15(g/mL)、乙醇浓度60%、70℃提取60 min,总酚提取率10.21%。提取液对自由基清除能力及铁离子还原力均高于VC溶液,对DPPH自由基的清除效果最好,浓度为20μg/mL的提取液对DPPH自由的清除率可达95%。说明酚类提取液体外抗氧化能力较强。

:The extraction and antioxidation of phenolic compounds in Cabernet Sauvignon pomace were studied.And that laid a foundation for the development and utilization of its resources.The total phenol extraction rate was used as the evaluation index,single factor experiments and orthogonal experiments were used to optimize the extraction conditions.VC was used as a reference for determining the ABTS+Free radicals,DPPH Free radicals,hydroxyl free radical scavenging and iron ion reducibility of the total phenol extract.The results showed that under the same conditions,the extraction rate of tannins and phenols from grape seeds of Cabernet Sauvignon was higher than that of grape skins.The optimum conditions were extracted under the ratio of material to liquid 1:15(g/mL),ethanol concentration 60%,extraction at 70℃for 60 min,and the total phenolic compounds extraction rate was 10.21%.The scavenging ability of the extracts to free radicals and iron ion was higher than that of VC solution,and the DPPH radical scavenging effect was best.The clearance rate could reach 95%when the extract solution was 20μg/mL,which indicated a strong in vitro antioxidant capacity of grape pomace extraction.