脑卒中患者MTHFR基因多态性与同型半胱氨酸水平的关系研究

Correlation between MTHFR Gene Polymorphism with Homocysteine Level in Patients with Ischemic Stroke

目的探讨缺血性脑卒中患者5,10-亚甲基四氢叶酸还原酶(MTHFR)基因多态性与血浆同型半胱氨酸(Hcy)水平的关系。方法选择2017年1月—2017年12月收治的120例缺血性脑卒中患者作为观察组,同期健康体检者120例作为对照组。对两组血浆Hcy水平和MTHFR基因677位点的等位基因进行观察。结果观察组收缩压和Hcy水平高于对照组(P <0. 05)。两组MTHFR基因野生型(C/C)、杂合突变型(C/T)和纯合突变型(T/T)分布差异有统计学意义(P <0. 01)。观察组CT+TT频率高于对照组(P <0. 05)。两组T/T型的Hcy水平均高于本组C/C和C/T型(P <0. 05)。观察组T/T和C/T型Hcy水平高于对照组(P <0. 05)。结论 Hcy参与缺血性脑卒中的发生,MTHFR的C677T位点基因突变可能是缺血性脑卒中的危险因素。

Objective To investigate correlation between 5,10-methylenetetrahydrofolate reductase(MTHFR)gene polymorphism with plasma homocysteine(Hcy)level in patients with ischemic stroke.Methods A total of 120 patients with ischemic stroke admitted from January to December 2017 were selected as observation group,and other 120 healthy people taking physical examination at the same period were selected as control group.Plasma Hcy levels and allele at the 677 locus of MTHFR gene were observed in two groups.Results Values of systolic blood pressure and Hcy levels in observation group were significantly higher than those in control group(P<0.05).There were significant differences in distributions of wild type(C/C),heterozygous mutant(C/T)and homozygous mutant(T/T)of MTHFR gene between two groups(P<0.01).Frequency of CT+TT in observation group was more than that in control group(P<0.05).Hcy levels of T/T were significantly higher than those of C/C and C/T in the same group in two groups(P<0.05).HCY levels of C/C and C/T in observation group were significantly higher than those in control group(P<0.05).Conclusion HCY is involved in pathogenesy of ischemic stroke,and MTHFR C677T gene mutation may be a risk factor for ischemic stroke.