日本血吸虫抗独特型单克隆抗体NP30免疫后小鼠血清IFN-γ、IL-4水平的变化

Changes of serum IFN-γ and IL-4 level in mice immunized with anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum

目的观察日本血吸虫抗独特型单克隆抗体NP30免疫后机体血清有关细胞因子水平的变化,初步探讨NP30保护性免疫的机制。方法雌性BALB/c小鼠经腹腔注射免疫NP30 10μg/次,共免疫3次,每次间隔2w。同时设PBS对照组。末次免疫1w后开始尾静脉采血,连续采血6次,每次间隔1w。夹心ELISA法测血清中细胞因子IFN-γ、IL-4的水平。结果与PBS对照组相比,10μgNP30免疫组血清IFN-γ水平增高,且以第2w(P=0. 035)、第3w(P=0. 019)最明显; NP30免疫组血清IL-4水平也增高,且以第3w(P=0. 028)最明显。结论从结果可以看出INF-γ水平升高出现较早,但维持时间较短,而IL-4水平升高出现较晚,但维持时间较长。说明NP30的保护性免疫早期以细胞免疫为主要类型,而在后期则转为体液免疫为主要类型。

Objective To investigate the protective immunity induced by the anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum in mice.Methods Twenty female BALB/c mice were equally randomized into experimental group and control group.Mice in the experimental group were intraperitoneally injected with 10μg NP30 by 3 doses every other two weeks,and those in the control group were intraperitoneally treated with PBS.Caudal vein blood was taken one week after final immunization,every one week in a total of 6 times.Sandwich-ELISA was performed to detect the expression of serum IFN-γand IL-4.Results IFN-γand IL-4 levels were higher in experimental group than in control group,with significantly increased IFN-γseen at week 2 and 3,and IL-4 level at week 2 following immunization(P=0.035,P=0.019,respectively).Conclusion Early elevated yet shortly lasted INF-γlevel and delayed IL-4 expression were seen in mice induced by anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum,suggesting that cell-mediated immunity plays a major role in the early stage,whereas immunity functions in later stage in the immune response of mice to this antibody.