基于自组装法制备茶多酚-透明质酸纳米粒工艺研究

Preparation of tea polyphenol-hyaluronic acid nanoparticles based on self-assembly

为提高茶多酚产品稳定性,更好发挥茶多酚在日化领域的功效价值,采用PEG化的水溶性高分子透明质酸(HA)为载体,通过自组装纳米结构包载茶多酚,形成纳米粒稳态体系,并对产品微观形态及离心稳定性进行了考察。结果表明,随着HA浓度增加,茶多酚纳米粒的粒径逐渐增大,而随着茶多酚单体EGCG添加量的增加,茶多酚纳米粒粒径呈先增后减的趋势。各因素对样品粒径影响程度的大小顺序为:m PEG-NH2添加量> EGCG添加量>透明质酸钠(HA)浓度。在HA浓度0. 02%(质量分数)、m PEG-NH2添加量0. 03 g,EGCG溶液添加量420μL条件下,体系具有最佳离心稳定性。冻干茶多酚粒子微观结构由丝状及颗粒状微粒相互包覆缠绕而成,呈三维网络结构。

In order to improve the stability of tea polyphenols and give full play to the value of tea polyphenols in the field of daily chemicals,PEGylated water-soluble macromolecular hyaluronic acid was used as a carrier to encapsulate tea polyphenols through self-assembled nanostructures,and the microscopic morphology and centrifugal stability of the products were investigated.The results showed that with the increase of HA concentration,the particle size of tea polyphenols nanoparticles gradually increased.With the increase of EGCG dosage,the particle size of tea polyphenol nanoparticles first increased and then decreased.The order of the influence degree of each factor on the particle size of the sample is:mPEG-NH 2 addition>EGCG addition>HA concentration.The system has the best centrifugal stability under the conditions of HA concentration 0.02%,mPEG-NH 2 addition 0.03 g,and EGCG addition 420μL.It was found that the micro-structure of the freeze-dried tea polyphenols was entangled with filamentous and granular particles and formed a three-dimensional network structure.