摘要
研究了蒙山莴苣总黄酮的纯化条件和体外抗氧化能力。结果表明,最佳的聚酰胺柱层析纯化条件为:用p H 5～6,质量浓度5. 21 mg/m L的上样液1. 5 BV,按1. 5 m L/min上样,用4 BV水淋洗,4. 5 BV 80%乙醇溶液(V/V)按1. 5 m L/min解吸,聚酰胺每使用5次可以进行一次再生。以VC为对照,蒙山莴苣总黄酮对DPPH自由基、·O-2和·OH都有一定的清除能力,对Fe3+有一定的还原能力,但效果均弱于VC。
The purification and the antioxidant activity in vitro was evaluated by artificial free radicals in detail.The result showed that the optimal extraction conditions were as follows:the concentration of sample solution was 5.21 mg/mL,pH was 5~6,adsorption rare was 1.5 mL/min,sample volume was 1.5 BV,water washing resin column with 4 BV,the elution rate was 1.5 mL/min with 4.5 BV 80%ethanol had a best effect with polyamides for 5 times.The total flavonoids in Lactuca tatarica had a certain scavenging effect on DPPH radicals,superoxide anion radicals and hydroxyl radicals.And the extract also showed a certain reduction ability which were all a little lower than that of ascorbic acid.In general,total flavonoids from Lactuca tatarica had a good antioxidant activity in vitro.
作者
王菲
侯雨歌
李洪影
田超
杨磊
WANG Fei;HOU Yu-ge;LI Hong-ying;TIAN Chao;YANG Lei(College of Chemistry,Chemical Engineering and Environmental Engineering,Liaoning Shihua University,Fushun 113001,China)
出处
《应用化工》
CAS
CSCD
北大核心
2018年第11期2423-2427,共5页
Applied Chemical Industry
基金
辽宁省教育厅科学研究一般项目资助(L2016006)
关键词
蒙山莴苣
总黄酮
纯化
体外抗氧化活性
Lactuca tatarica
total flavonoids
extraction
antioxidant activity in vitro
