Anisopus mannii叶的化学成分和抑制黑色素生成活性研究

Chemical constituents from the leaves of A nisopus m annii and the melanogenesis inhibitory activities

采用硅胶、ODS和大孔树脂等柱色谱分离方法对Anisopus mannii的叶进行分离纯化,通过NMR、HR-ESI-MS等波谱技术鉴定了7个化合物分别为:3β-acetoxylup-20(29)-ene (1),1-acetoxy-2-isopropyl-1-tridecene (2),rutin (3),3,6'-diferuloylsucrose (4),3-O-β-D-glucopyranosyl-3-β-hydroxyolean-12-en-28-oic acid 28-O-[α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranosyl] ester (5),conduritol A (6),hoyacarnoside I (7)。同时采用B16细胞为黑色素抑制的筛选模型,筛选了所有化合物的黑色素抑制活性和细胞毒性。结果显示:所有化合物均具有不同程度的抑制黑色素生成的活性,其中化合物5显示出最优活性[30μmol/L给药浓度,黑色素含量:(27. 4±3. 5)%,细胞存活率:(54. 9±5. 6)%],可以作为美白剂进一步开发。

The phytochemical studies on the leaves of Anisopus mannii led to the isolation of seven compounds by silica gel,ODS,DIAION HP-20,Sephadex LH-20colunmn chromatographer,their structures were elucidated on the basis of the spectroscopic analyses(NMR,HRMS)and the comparisons with the literatures as3β-acetoxylup-20(29)-ene(1),1-acetoxy-2-isopropyl-1-tridecene(2),rutin(3),3,6′-diferuloylsucrose(4),3-O-β-D-glucopyranosyl-3-β-hydroxyolean-12-en-28-oic acid28-O-[α-L-rhamnopyra-nosyl-(1→2)-β-D-glucopyranosyl]ester(5),conduritol A(6),hoyacarnoside I(7).Meanwhile,the isolated compounds were evaluated for their inhibitory activities against melanogensis in B16melanoma cells,as the results,all compounds exhibited melanogenesis-inhibitory activity and compound5showed a strongest activity(Melanin content:(27.4±3.5)%,Cell Viability:(54.9±5.6)%with a concentration of30μmol/L)which could be further developed.