ZnO薄膜的sol-gel法制备及其对腐败希瓦氏菌生物被膜的抑制性能

Preparation of Zinc Oxide Films by Sol-Gel Method and Their Anti-Biofilm Properties against Shewanella putrefaciens

借助真空条件,采用溶胶-凝胶(sol-gel)法在孔径约为50 nm的多孔钛片上制备具有不同微观形貌的ZnO薄膜,并对其进行X射线衍射光谱、红外光谱、扫描电子显微镜、透射电子显微镜、选区电子衍射、亲水性表征分析,研究ZnO薄膜微观结构对其抑制腐败希瓦氏菌生物被膜性能的影响。结果表明,ZnO为六方纤锌矿型,溶胶涂膜次数影响ZnO薄膜的微观形貌。溶胶涂膜1次所得薄膜表面沉积的ZnO总量较小,且ZnO首先在TiO2孔的边缘沉积,溶胶涂膜4次所得薄膜上ZnO沉积量增大,沿孔道顶端密集堆积。多孔钛片呈疏水性,ZnO薄膜呈亲水性,且随涂膜次数增多,其亲水性增强。由于多孔钛片具有疏水性,其抑制水产品腐败希瓦氏菌生物被膜的性能高于普通钛片;由于ZnO具有杀菌性能,则ZnO薄膜对生物被膜的抑制性能增强,且涂膜4次的样品对生物被膜的抑制性能更优。

ZnO films with different morphologies were prepared by the sol-gel method under vacuum conditions on the surface of porous titanium sheets with pores of 50 nm,and the thin films were characterized by X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR),scanning electron microscope(SEM),transmission electron microscope(TEM),selected area electron diffraction(SAED)and hydrophily.The effect of the morphology of ZnO films on their antibiofilm properties against the food spoilage bacterium Shewanella putrefaciens was determined.The results showed that ZnO had a hexagonal wurtzite structure,and the microstructure of ZnO thin films was affected by the number of coating cycles.The amount of ZnO deposited on the film with one cycle of coasting was low,and ZnO was firstly deposited on the edge of TiO2 porous.When the sol was deposited for 4 times,the amount of ZnO deposited was increased,and ZnO was packed densely along the top of channels.The porous titanium films were hydrophobic,and they became hydrophilic after the deposition of ZnO.The hydrophilicity of the films was strengthened with increasing number of coating cycles.As the porous titanium plate was hydrophobic,its anti-biofilm property against S.putrefaciens was stronger than that of ordinary titanium plate.However,because of the antibacterial properties of ZnO,the antibiofilm properties of the ZnO films were enhanced by sol-gel coating,and the ZnO film with 4 cycles of coating possessed the best antibiofilm properties.