不同保鲜液对秋石斛切花生理效应的影响

Effects of Different Fresh-keeping Agents on Physiological Characters of Dendrobium hybridus Cut Flowers

为筛选适宜秋石斛鲜切花的保鲜配方,以秋石斛鲜切花为研究对象,采用6种保鲜液配方(以蒸馏水为对照)对其进行瓶插处理,测定秋石斛鲜切花的生理生化指标,研究不同保鲜液对秋石斛切花生理效应的影响。结果表明:随着瓶插时间的增加,秋石斛切花超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、脯氨酸(Pro)含量、可溶性蛋白和可溶性糖的总体趋势均先增高后下降。综合比较发现配方3(3%蔗糖+150mg·L^(-1)柠檬酸+40mg·L^(-1) AgNO_3)既能延长秋石斛的瓶插期,达到19d,比对照长了8d,又能较好地保持其花瓣形态及色泽、促进花茎的增大,保鲜效果最佳。

In order to screen out the best formulation for fresh cut flowers of Dendrobium hybridus,we used the cut flowers of Dendrobium hybridus as the research object,and utilized six different fresh-keeping agents(distilled water as control)in their preservation,determined the physiological and biochemical indexes of the fresh cut flower,studied the effects of different fresh-keeping agents solutions on the physiological effects of Dendrobium hybridus cut flowers.The results showed that with the increase of vase time,superoxide dismutase(SOD),peroxidase(POD)activities,catalase(CAT)activity,proline(Pro)contents,soluble protein and soluble sugar of Dendrobium hybridus cut flowers increased firstly and then declined;Based on the compre-hensive analysis of the morphological,physiological and biochemical characteristics of Dendrobium hybridus cut flowers,it was found that No.3treatment,consisted of3%Sugar+150mg·L-1citric acid+40mg·L-1AgNO3which was had the best effect among all the tested ones.Not only can it extend the vase life to19d,which was8d longer than the control in their vase life;but also maintain the shape and color of petals,promote the increase of stems,and have the best preservation effect.