摘要
组织蛋白酶B是肿瘤侵袭和转移密切相关的一种蛋白酶,研究其作用网络对于开发基于组织蛋白酶B的抗肿瘤药物非常重要。采用酵母双杂交技术构建相关文库,以人组织蛋白酶B作为诱饵蛋白质,寻找其在肿瘤细胞中的直接相互作用蛋白质。在84个初始阳性克隆中,共有15个能通过3个报告基因的检测。BLAST分析发现,在GenBank数据库中可以匹配7个基因,编码7种已知的蛋白质:BCL2-associated athanogene 6 (BAG6), filaminA,alpha(FLNA),ras homolog family member G (RHOG),metallothionein 2A (MT2A), ZXD family zinc finger C(ZXDC),DEP domain containing 7(DEPDC7),A kinase(PRKA) interacting protein 1(AKIP1)。
Cathepsin B is an enzymatic protein that was closely related with tumor cell invasion and metastasis.The network of Cathepsin B is very important for research and development of new anticancer drug,for example,new Cathepsin B inhibitor.Using yeast two hybrid technique,the proteins that interacting with Cathepsin B were identified.Results:After screening analysis,15 positive clones of putative interactions were obtained and they were identified by sequencing and utilizing of the data online(http://www.ncbi.nlm.nih.gov).There were 7 genes including BCL2-associated athanogene 6(BAG6),filamin A,alpha(FLNA), ras homolog family member G(RHOG), metallothionein 2A(MT2A), ZXD family zinc finger C(ZXDC), DEP domain containing 7(DEPDC7),A kinase(PRKA)interacting protein 1(AKIP1).
作者
王道余
赵胜娟
刘燕
刘淼
冯利兴
WANG Daoyu;ZHAO Shengjuan;LIU Yan;LIU Miao;FENG Lixing(School of Life Science,Fudan University,Shanghai 200433,China;College of Food&Bioengineering,Henan University of Science and Technology,Luoyang 471023,China;Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2018年第12期1255-1260,共6页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(81373964)
国家科技重大专项项目(2014ZX09301-306-03)
上海科技支撑项目(13431900401)
上海科技创新行动计划项目(15140904800)