新疆塔城牧区原料乳中酵母菌的分离鉴定

Separation and Identification of Yeast from Raw Milk in Tacheng of Xinjiang

为研究新疆塔城牧区原料乳中酵母菌的分布情况,从该地区采集了7份原料乳样品,通过传统培养方法,采取形态学、生理生化特性鉴定、5.8S rDNA序列同源性分析相结合的方法,分离得到33株酵母菌,共鉴定出6个属8个种,其中20株库德毕赤酵母Pichia kudriavzevii(61%)为优势菌株;2株发酵毕赤酵母Pichia fermentans(6%);3株美极梅奇酵母Metschnikowia pulcherrima (9%);2株乳酸克鲁维酵母Kluyveromyces lactis (6%);2株马克思克鲁维酵母Kluyveromyces marxianus(6%);2株胶红酵母Rhodotorula mucilaginosa(6%);1株葡萄有孢汉逊酵母Hanseniaspora uvarum(3%);1株单孢酿酒酵母Kazachstania unispora(3%)。研究表明,该地区原料乳中的酵母菌多样性较好,有其独特的酵母菌资源,这为研究本地区的微生物资源提供了基础资料。

To study the distribution of yeast in raw milk in pastoral areas of Tacheng,Xinjiang.Seven samples of raw milk were collected from the areas.Using the traditional culture-dependent method,the isolation yeasts were identified according to the colonial morphology,biological properties and 5.8S rDNA sequence homology analysis.A total of 8 species belonging to 6 genera were identified from 33 strains isolated from the isolates,which were 20 strains of Pichia kudriavzevii(61%)were the dominant strains,2 strains Pichia fermentans(6%);3 strains Metschnikowia pulcherrima(9%);2 strains Kluyveromyces lactis(6%);2 strain Kluyveromyces marxianus(6%);2 strains Rhodotorula mucilaginosa(6%);1 strain Hanseniaspora uvarum(3%);1 strain Kazachstania unispora(3%).The study showed that the yeast diversity of raw milk in this region was good,and has its unique yeast resources.This provides the basic datas for the study of the microbial resources in this area.