酸解氧化魔芋葡甘聚糖对齐口裂腹鱼肠道微生物的影响

Effects of Dietary Acidolysis Products of Oxidized Konjac Glucomannan on Intestinal Microflora of Schizothorax prenanti

探讨了氧化魔芋葡甘露聚糖酸解产物对齐口裂腹鱼肠道微生物的影响。选取平均体质量为81±2.64 g健康齐口裂腹鱼360尾,随机分为4组(每组3个重复,每个重复30尾),分别为对照组(饲喂基础饲料)、LOKGM-0.8%组(基础饲料+0.8%LOKGM)、LOKGM-1.6%组(基础饲料+1.6%LOKGM)和LOKGM-3.2%组(基础饲料+3.2%LOKGM),试验期75 d(驯养期15 d,正试期60 d)。采用变性梯度凝胶电泳(DGGE)技术对齐口裂腹鱼后肠肠道内容物细菌16S rDNA V3可变区进行菌群多样性分析,利用实时荧光定量PCR技术对肠道中嗜水气单胞菌及芽孢杆菌的数量进行定量分析。结果表明:与对照组相比,LOKGM-1.6%与LOKGM-3.2%组后肠肠道菌群多样性显著增加(P<0.05),LOKGM-0.8%组无显著影响(P>0.05);LOKGM-0.8%组齐口裂腹鱼前肠和中肠嗜水气单胞菌的数量显著减少(P<0.05),前肠芽孢杆菌的数量显著增加(P<0.05);LOKGM-1.6%组前中后肠嗜水气单胞菌数量均显著减少(P<0.05),中肠与后肠芽孢杆菌数量显著增加(P<0.05);LOKGM-3.2%组前肠和后肠嗜水气单胞菌的数量显著减少(P<0.05),前肠和中肠芽孢杆菌数量显著增加(P<0.05)。由此得出:氧化魔芋葡甘露聚糖酸解产物能够改善齐口裂腹鱼的肠道菌群结构,有利于保持鱼体健康。

To study the effects of dietary acidolysis products of oxidized konjac glucomannan(OKGM)on intestinal microflora of Schizothorax prenanti.A total of three hundred and sixty healthy fishes with average body weight of 81±2.64 g were randomly divided into 4 groups with 3 replicates and 30 fishes per replicate.The 4 groups were control group(basal diet)、LOKGM-0.8%group(basal diet+0.8%LOKGM)、LOKGM-1.6%group(basal diet+1.6%LOKGM)、LOKGM-3.2%group(basal diet+3.2%LOKGM),respectively.The experiment lasted for 75 d(15 d for adaptation and 60 d for test).Diversity of posterior gut microflora was determined based on V3 variable regions of bacteria 16S rDNA by using denaturing gradient gel electrophoresis(DGGE)technique.The number of Aeromonas hydrophila and Bacillus in the intestinal was quantified by real-time quantitative PCR.The results showed as follows:compared with the control group,the diversity of posterior intestinal microflora was significantly increased in the LOKGM-1.6%and LOKGM-3.2%group(P<0.05),but the diversity of posterior intestinal microflora was not affected in the LOKGM-0.8%group(P>0.05);the number of Aeromonas hydrophila in anterior and middle intestinal of Schizothorax prenanti was significantly decreased in the LOKGM-0.8%group(P<0.05),also the number of Bacillus in anterior intestinal was significantly increased(P<0.05);the number of Aeromonas hydrophila in anterior,middle and posterior intestinal was all significantly decreased in LOKGM-1.6%group(P<0.05)and the number of Bacillus in middle and posterior intestinal was significantly increased(P<0.05);the number of Aeromonas hydrophila in anterior and posterior intestinal was significantly decreased in LOKGM-3.2%group(P<0.05),besides,the number of Bacillus in anterior and middle intestinal was significantly increased(P<0.05).In conclusion,acidolysis products of oxidized konjac glucomannan can improve the configuration of posterior intestinal microflora of Schizothorax prenanti and keep the fish healthy.