心肌声学造影视觉评估兔急性心肌梗死后的微循环

Myocardial contrast visual assessment of microcirculation after acute myocardial infarction in rabbits

目的在兔急性心肌梗死模型上应用心肌声学造影视觉评估方法评价急性心肌梗死后梗死节段的血流灌注状况。方法将20只清洁级日本大耳兔随机分组,建立急性心肌梗死模型组(A组)和假手术对照组(S组)。采集术前及术后10、45 min心电图,并在术前及术后20 min、2 h、6 h进行常规超声及超声造影,综合评估分析兔急性心肌梗死后造模节段血流灌注情况。实验结束后取出造模节段心肌组织进行病理学检查。结果常规超声心动图均表明缺血后不同时间点造模节段运动幅度显著改变,心肌组织学有显著改变,表明成功建立了日本大耳兔急性心肌梗死模型;同时心肌声学造影视觉评估表明A组造模节段心肌血容量及灌注速度均显著低于非梗死节段及S组对应节段,与常规超声心动图和病理组织学的检查结果一致。结论心肌声学造影的视觉评估能够准确判定急性心肌梗死后梗死心肌节段的血流灌注的状况,是一种有效的诊断手段。

Objective In a rabbit model of acute myocardial infarction,blood perfusion of the infarction segment after acute myocardial infarction was subjected to myocardial acoustic contrast visual evaluation.Methods Twenty cleangrade Japanese rabbits were randomly assigned to an acute myocardial infarction model group(group A)and a sham operation control group(group S).Preoperative and10-and45-min postoperative electrocardiograms(ECGs)were collected,and routine ultrasound and ultrasonic imaging were performed at20min,2h,and6h before and after the operation.Blood perfusion in the molding segment after acute myocardial infarction was comprehensively evaluated.At the end of the experiment,the myocardial tissue of the molding section was excised for pathological examination.Results Conventional echocardiography showed significant changes in the magnitude of the motion of the modular segment at different time points after ischemia,and significant changes in cardiac histology,indicating the successful establishment of the acute myocardial infarction model in Japanese rabbits.At the same time,visual evaluation of myocardial contrast sonography showed that the myocardial blood volume and perfusion speed of group A were significantly lower than those of the noninfarcted segments and the corresponding segments of group S,which was consistent with the result of routine echocardiography and pathological histology.Conclusion Visual evaluation of myocardial sonography can accurately determine the status of blood perfusion in the myocardial segment after acute myocardial infarction,which is an effectivediagnostic method.