维生素D受体基因敲除小鼠品系的建立与鉴定

Strain establishment and identification of vitamin D receptor gene knockout mice

目的探讨维生素D受体(VDR)基因敲除小鼠的最优繁育方式与鉴定方法,为进一步研究VDR功能及相关疾病提供动物模型。方法针对课题组前期构建的VDR敲除小鼠,采用4种不同的交配方式产生子代小鼠,统计子代的表型和基因型,比较VDR基因敲除纯合子(VDR^(-/-))和杂合子(VDR^(+/-))小鼠的繁殖能力,同时在mRNA和蛋白水平分析VDR表达情况。结果 VDR^(-/-)小鼠与VDR^(+/+)小鼠在表型上具有显著差异,主要表现在脱毛、体型小和活动能力下降等方面;4种繁育方式所产子代小鼠的基因型分布基本符合孟德尔遗传定律;VDR^(-/-)互交方式小鼠的生殖能力差,不能直接用于扩大VDR^(-/-)敲除鼠品系,而VDR^(+/-)互交方式可成功获得VDR^(-/-)小鼠,并且检测到VDR基因敲除性状能稳定遗传至子代小鼠,因此该方法是扩大品系的最佳途径。结论本实验成功鉴定出子代小鼠基因型并建立VDR基因敲除小鼠品系,为后续VDR功能研究奠定基础。

Objective This study aimed to investigate method for the optimal breeding and identification of vitamin D receptor(VDR)knockout mice,which could provide animal models for further research on VDR function and related diseases.Methods Based on VDR knockout mice previously obtained in our lab,four different mating methods were used to generate offsprings,the phenotypes and genotypes of which were evaluated and the reproductive ability of VDR knockout homozygous(VDR-/-)and heterozygous(VDR+/-)mice were compared.And,VDR expression was analyzed at the mRNA and protein levels.Results Compared with VDR+/+mice,VDR-/-mice had a significantly different phenotype,mainly in terms of having less hair,small size,and decreased mobility.The genotype distributions of the offspring mice produced by the four breeding method were basically in accordance with Mendel’s law of inheritance.VDR-/-cross-over had poor reproductive ability and could not be directly used to expand VDR-/-homozygous knockout mouse strains,while VDR+/-cross-over could successfully obtain VDR-/-mice.It was also determined that VDR knockout traits could be stably inherited by offspring mice,so VDR+/-cross-over is the best way to expand the strains.Conclusions This experiment has successfully identified the genotypes of offspring mice and established VDR knockout mouse strains,laying the foundation for subsequent research on VDR function.