摘要
南荻(Miscanthus lutarioriparius)是中国特有的芒属植物,是极具应用前景的纤维类生态能源草。为研究南荻多倍体在NaCl胁迫下的无机盐离子吸收特征,本研究以南荻多倍体和奇岗为研究对象,测定其在0、0.2%、0.5%、0.8%NaCl胁迫下对K+、Ca2+、Mg2+、SO42-和NO3-的吸收利用率,并同时测定各试验材料的存活率。结果表明,各南荻多倍体对K+、Ca2+、Mg2+和NO3-的吸收率普遍随NaCl浓度的增加呈上升趋势,对SO42-的吸收率除多倍体L7、L8和L9随NaCl浓度的增加有明显的上升以外,其他材料均受到不同程度的抑制;而作为对照的奇岗,总存活率较低(38.89%),对K+、Ca2+、Mg2+和SO42-的吸收均受到NaCl的抑制,对NO3-的吸收率则在高NaCl浓度下有所增加;在0.8%NaCl胁迫下,南荻三倍体L8和L9存活率较高(66.67%),且L8和L9对Ca2+、Mg2+、SO42-和NO3-的吸收率与其对照相比增幅均高于其他材料;南荻三倍体L2和L4在0.8%NaCl胁迫下存活率最低(16.67%),且L2和L4对各无机离子的吸收率与对照相比增幅均低于其他材料。通过本研究,获得了南荻通过调节对K+、Ca2+、Mg2+、SO42-和NO3-的吸收量响应NaCl胁迫的初步数据,为研究NaCl胁迫下南荻的无机渗透调节生理响应机制奠定基础。
Miscanthus lutarioriparius is a species of Miscanthus domestic to China and is an ecological grass with a promising future.To study the inorganic osmotic response of M.lutarioriparius polyploids under NaCl stress,M.lutarioriparius polyploids and Miscanthus×giganteus were treated with0,0.2%,0.5%,and0.8%NaCl solution.The absorption rate of K^+,Ca^2+,Mg^2+,SO4^2-,and NO3^-were analyzed and the survival rate of each test line was measured.The results showed that the absorption rates of K^+,Ca^2+,Mg^2+,and NO3^-by each polyploid of M.lutarioriparius generally increased with the increase in NaCl concentration,and the absorption rate of SO4^2-except for L7,L8,and L9increased significantly with the increase in NaCl concentration.Other tested lines were inhibited to varying degrees.As a control,Miscanthus×giganteus(G)had a lower survival rate(38.89%),its absorption of K^+,Ca^2+,Mg^2+,and SO4^2-was inhibited by NaCl,and the absorption rate of NO3^-increased at high NaCl(0.8%)concentration.After treatment with0.8%NaCl solution,the survival rate of M.lutarioriparius polyploids(L8and L9)were the highest(66.67%),and the absorption rates of Ca^2+,Mg^2+,SO4^2-,and NO3^-by L8and L9were higher than those of other lines compared with the control.The survival rate of M.lutarioriparius polyploids(L2and L4)were the lowest(16.67%)under0.8%NaCl solution,and the absorption rates of L2and L4to each inorganic ion were lower than that of the control.From this study,the preliminary data of M.lutarioriparius was obtained,by adjusting the absorption of K^+,Ca^2+,Mg^2+,SO4^2-,and NO3^-in response to NaCl stress.This provides an opportunity for further studying of the physiological response mechanism of inorganic osmotic adjustment of M.lutarioriparius under NaCl stress.
作者
段钧译
石艳兰
林宇环
黄红梅
刘清波
易自力
陈智勇
DUAN Junyi;SHI Yanlan;LIN Yuhuan;HUANG Hongmei;LIU Qingbo;YI Zili;CHEN Zhiyong(College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, Hunan, China;Hunan Engineering Laboratory of Miscanthus Ecological Application Technology, Changsha 410128, Hunan, China)
出处
《草业科学》
CAS
CSCD
2018年第12期2893-2902,共10页
Pratacultural Science
基金
国家自然科学基金"芒属植物异源多倍体表型变异与基因差异表达的相关性研究"(31471557)
关键词
南荻
奇岗
芒属
能源植物
多倍体
NACL胁迫
无机盐离子
Miscanthus lutarioriparius
Miscanthus×giganteus
Miscanthus
absorption
polyploid
NaCl stress
inorganic ions