TaGSO1的克隆及其在叶锈菌侵染TcLr26过程中的表达分析

Cloning of TaGSO1 and its expression in the process of infecting TcLr26 by Puccinia triticina

为了挖掘新的小麦抗叶锈菌相关基因,本试验利用cDNA末端快速扩增(rapid amplification of cDNA ends,RACE)技术从小麦抗叶锈近等基因系TcLr26中克隆得到抗病相关基因TaGSO1,对其进行生物信息学分析,并对其在小麦与叶锈菌互作过程中的转录水平表达模式进行检测。分析结果显示,该基因全长2598bp,编码866个氨基酸,其蛋白结构包含7个LRRs结构域和1个跨膜区以及1个短的细胞质结构,属于典型的LRR-RLPs受体蛋白;系统发育分析表明,TaGSO1基因与来自乌拉尔图小麦的EMS54105.1基因亲缘关系最近。RT-qPCR分析结果表明,该基因在TcLr26与叶锈菌的不亲和互作过程其表达量随接种时间延长明显升高,而在亲和组合中该基因的表达量很低,并且随着侵染时间的延长表达量几乎没有变化。外施SA发现TaGSO1的表达受SA诱导,推测该基因可能介导了SA信号传导途径。

In order to find new genes related to Puccinia triticina resistance in wheat,this study used the rapid amplification of cDNA ends(RACE)technique to clone the resistant gene TaGSO1 from wheat leaf rust near-isogenic line TcLr 26.It carried out bioinformatics analysis and analyzed the transcriptional level expression pattern in wheat with P.triticina interactions.The results showed that the full length cDNA of the TaGSO1 gene was 2598 bp and it encoded 866 amino acids.Its protein structure consists of seven LRRs domains and a transmembrane region and a short cytoplasmic structure,which was a typical LRR-RLPs receptor protein.The phylogeny analysis showed that TaGSO1 gene was closely related to EMS54105.1 gene from Triticum urartu Thum.ex Gandil.The results of RT-qPCR analysis showed that the expression level of this gene in TcLr 26 with P.triticina increased significantly in the incompatible combination with the inoculation time.But the expression level of the gene in the affinity combination was low,and there was almost no change in the prolonged expression of the infection time.The application of SA revealed that the expression of TaGSO1 was induced by SA.It was speculated that this gene may mediate the SA signaling pathway.