摘要
为了获取高效的病死猪堆肥降氨除臭微生物菌株,利用氨气选择性培养基定性初筛和硼酸吸收法复筛相结合的方法,分离筛选高效降氨微生物,并对其菌种分类进行了鉴定。从猪粪、土壤、病死猪堆肥等样品中共分离筛选获得7株对NH3具有降解效果的菌株,分别编号为YX-1、YX-2、YX-3、YX-4、YX-6、YX-7、YX-8。在30 d模拟堆肥周期中,菌株YX-3对氨气的去除效率最高,达56. 9%。根据形态学特征、生理生化特征及16S r DNA序列分析,该菌鉴定为暹罗芽孢杆菌(Bacillus siamensi)。通过单因素试验,确定菌株YX-3降氨除臭的最佳温度为30~40℃,最佳接种量为8%。菌株YX-3对H2S的去除能力显著低于对氨气的去除能力,说明该菌去除臭气成分的功能专一。筛选获得的菌株YX-3丰富了堆肥除臭微生物资源库,在农业环保领域具有潜在的应用价值。
In order to obtain deodorant microorganisms for dead-pig composting,effective strains were screened by combining NH 3 selective media and the boric acid absorption method and the phylogenetic position of YX-3 was determined.Seven strains with ammonia emission ability were sceened from pig compost,soil and dead-pig compost samples,labeles as YX-1、YX-2、YX-3、YX-4、YX-6、YX-7、YX-8.In a composting experiment of 30 d,the NH 3 emission rate of strain YX-3 was 56.9%,which was higher than other six strains.Based on the results of morphology,physio-biochemical tests and 16S rDNA sequence analysis,strain YX-3 was identified as Bacillus siamensi.The single-factor test was used to determine the optimum deodorant conditions of YX-3.The optimum culture temperature was 30~40℃and the best inoculation amount was 8%.Compared with the NH 3 emission rate,YX-3 showed lower H 2S emission ability,which indicated that efficient H 2S emission strains should be screened for high deodorizing effect.The result shows that Bacillus siamensi YX-3 has great potential application prospects in the field of agriculture and environment protection.
作者
刘标
尹红梅
刘惠知
LIU Biao;YIN Hong-mei;LIU Hui-zhi(Hunan Microbiology Institute, Changsha 410009, China)
出处
《科学技术与工程》
北大核心
2018年第34期248-252,共5页
Science Technology and Engineering
基金
湖南省科技厅重点研发计划(2017NK2155)
湖南省生猪产业技术体系生猪产业规模养殖与环境控制岗位项目资助
关键词
病死猪堆肥
降氨
除臭菌
菌种鉴定
dead-pig composting
ammonia emission
deodorant strain