不同亚油酸水平培养基对溶纤维丁酸弧菌脂肪酸合成的影响

Effects of Different Linoleic Acid Contents in Medum on the Synthesis of Fatty Acids of Butyrivibrio fibrisolvens

为探究亚油酸对反刍动物瘤胃中溶纤维丁酸弧菌脂肪酸合成的影响,试验用不同水平亚油酸培养基进行溶纤维丁酸弧菌体外厌氧培养试验,分光光度计测定菌液D600nm值达到1.0时,在培养基中添加亚油酸,使试验1组(对照)、2、3及4组培养基亚油的浓度分别为0、2.5、5.0及7.5mg/100mL,培养24h后收集菌液检测35种脂肪酸含量。结果显示,细菌代谢相关的C8∶0、C11∶0、C14∶0、C15∶0、C17∶0、C17∶1、C18∶1(n-9)C、C18∶2(n-6)C、C18∶3(n-6)、C20∶0、C20∶2、C20∶3(n-3)、C20∶3(n-6)、C20∶5(n-3)、C21∶0、C22∶0、C22∶2、C22∶1(n-9)及C22∶6(n-3)脂肪酸随着培养基中亚油酸浓度的提高无显著变化(P>0.05);培养基中添加亚油酸使C10∶0、C12∶0、C16∶0、C18∶0、C20∶4(n-6)、C23∶0、C24∶1合成量显著减少(P<0.05);而C15∶1及C18∶3(n-3)合成量在2.5mg/100mL亚油酸组显著增加(P<0.05);C14∶1在对照组中合成量最高,在添加亚油酸的试验组中,随着亚油酸含量增加而增加;C24∶0在7.5mg/100mL亚油酸组合成量最高。综上所述,亚油酸对于溶纤维丁酸弧菌合成脂肪酸有一定影响作用,可抑制C10∶0、C12∶0、C16∶0、C18∶0、C23∶0、C14∶1、C20∶4(n-6)等脂肪酸的合成,C15∶1、C16∶1、C18∶3(n-3)含量增加,但超过一定量时合成量会减少。

To explore the effect of linoleic acid on the synthesis of fatty acids of Butyrivibrio fibrisolvens in ruminant animals,different levels of linoleic acid medium were used to conduct an anaerobic culture test with Butyrivibrio fibrisolvens.Linoleic acid was added to the culture medium to make its concentrations in test groups 1(control group),2,3 and 4 were 0,2.5,5.0 and7.5 mg/100 mL when D600 nmvalue of bacteria reached 1.0.The bacteria fluid was collected and tested for 35 kinds of fatty acids after cultured for 24 h.The results showed that there were no significant difference in the contents of C8∶0,C11∶0,C14∶0,C15∶0,C17∶0,C17∶1,C18∶1(n-9)C,C18∶2(n-6)C,C18∶3(n-6),C20∶0,C20∶2,C20∶3(n-3),C20∶3(n-6),C20∶5(n-3),C21∶0,C22∶0,C22∶2,C22∶1(n-9)and C22∶6(n-3)with the increase of linoleic acid(P>0.05);The amount of C10∶0,C12∶0,C16∶0,C18∶0,C20∶4(n-6),C23∶0 and C24∶1 were significantly decreased response to the linoleic acid addition(P<0.05).The contents of C15∶1 and C18∶3(n-3)were significantly increased in 2.5 mg/100 mL linoleic acid group(P<0.05),the amount of C14∶1 in control group was the highest and that would increase with the linoleic acid increase.The amount of C24∶0 in 7.5 mg/100 mL linoleic acid group was the highest.In conclusion,linoleic acid had a certain effect on the synthesis of fatty acids of Butyrivibrio fibrisolvens,it could inhibit the synthesis of C10∶0,C12∶0,C16∶0,C18∶0,C23∶0,C14∶1 and C20∶4(n-6),increase the synthesis of C15∶1,C16∶1 and C18∶3(n-3)which would decrease when the linoleic acid content exceed a certain value.