不同热处理DDGS中CNCPS组分与糠氨酸含量的相关性研究

Study on the Correlation Between the CNCPS Fractions and Furosine Content in DDGS with Different Heat Treatment

本试验旨在利用康奈尔净碳水化合物-蛋白质体系(CNCPS)的原理和方法评定不同程度热处理玉米乙醇糟(DDGS)的营养价值,并探究CNCPS组分及其瘤胃降解特性和小肠消化率与美拉德反应中间产物糠氨酸之间的相关性,为饲料营养价值的评定提供新的研究方法和思路。本试验将湿DDGS进行不同温度(110、120、130℃)、不同时间(0.5、1.0、1.5h)的烘干处理,采用常规化学分析方法并结合CNCPS和AMTS-Cattle数据库对蛋白质各组分的瘤胃降解特性和小肠消化特性进行估测,同时采用高效液相色谱法测定糠氨酸的含量,进而探求CNCPS各组分瘤胃降解特性和小肠消化率与糠氨酸含量之间的相关性并建立回归方程。结果显示:(1)不同程度热处理的DDGS之间,糠氨酸含量、蛋白质组分、瘤胃可降解蛋白质组分、瘤胃不可降解蛋白质组分、小肠可消化蛋白质组分均存在显著差异(P<0.05)。(2)糠氨酸与蛋白质PA2、PB1、PB2和PC组分,瘤胃可降解蛋白RDPB2组分,瘤胃不可降解蛋白RUPB2、RUPC和TRUP组分,小肠消化蛋白IDGPB2组分间均存在显著相关性(P<0.05),并可通过回归建立预测方程。综合试验结果,经过不同程度热处理的DDGS的蛋白质组分和糠氨酸含量存在显著相关性,可以利用糠氨酸含量对不同程度热处理DDGS的蛋白质组分的瘤胃降解特性和小肠消化率进行估测。

The aim of this study was to evaluate the nutritional value of different degrees heattreated of corn dried distillers grains and solubles(DDGS)using the principle and method of Cornell Net Carbohydrate Protein System(CNCPS),and to explore the correlation of CNCPS components and its ruminal degradability and intestinal digestibility to furosine which was Maillard reaction intermediate.This study was respected to provide new methods and ideas for the evaluation of feed nutritional value.The wet DDGS were dried at different temperatures(110,120,130 ℃)for different times(0.5,1.0,1.5 h),the protein components and their ruminal degradability and intestinal digestibility were estimated by conventional chemical analysis combined with CNCPS and AMTS-Cattle database,and the furosine content was determined by high performance liquid chromatography.Then the correlation between ruminal degradability and intestinal digestibility of the CNCPS components and furosine content were researched and the corresponding regressionequations were established.The results showed that:(1)There were significant differences in furosine content,protein components,ruminal degradable,ruminal non-degradable and intestinal digestibility of protein components among DDGS treated with different degrees of heat(P<0.05).(2) There were significant correlation between furosine content and PA2,PB1,PB2 and PC components,RDPB2,RUPB2,RUPC and TRUP components,and IDGPB2 components(P<0.05).And the predictive equations could be established by regression.In summary,there were significant correlations between the protein components and furosine content of DDGS with different degrees of heat treatment,and the furosine content could be used to estimate the rumen degradability and intestinal digestibility of protein components in DDGS treated with different degrees of heat.