大鼠肾移植急性排斥反应过程中IL-17表达及其意义

Expression and significance of IL-17 in acute rejection of renal transplantation in rats

目的:探讨IL-17在大鼠肾移植急性排斥反应过程中的表达特点及其意义。方法:将大鼠随机分为正常对照组、同系基因对照组、同种异体移植组、免疫抑制剂干预组,并建立肾移植急性排斥反应模型,用RT-PCR及免疫组织化学方法检测急性排斥时IL-17在肾组织中的表达。结果:同种异体移植组在移植后第3天、5天和7天,移植肾IL-17均明显升高,与其他组相比,均有统计学意义(P<0. 05),其中以第5天为最高,差异具有统计学有意义(P<0. 05)。结论:IL-17参与了大鼠肾移植急性排斥反应的发生,并为早期事件,IL-17检测可能为人类肾移植早期急性排斥反应的诊断和治疗提供理论依据。

Objective:To investigate the expression characteristics and significance of IL-17in acute rejection of renal transplantation in rats.Methods:Rats were randomly divided into normal control group,homogeneic control group,allogeneic transplantation group and immunosuppressant intervention group,and acute rejection reaction model of renal transplantation was established.The expression of IL-17in renal tissue during acute rejection was detected by RT-PCR and immunohistochemistry.Results:IL-17was significantly increased in the allograft group on the3rd,5th and7th days after transplantation.Compared with other groups,IL-17was statistically significant(P<0.05),of which the5th day was the highest and statistically significant(P<0.05).Conclusion:IL-17is involved in the occurrence of acute rejection of renal transplantation in rats,and may provide theoretical basis for the diagnosis and treatment of acute rejection in early stage of human kidney transplantation.