稻秆发酵生产纤维素酶的基质预处理工艺研究

Substrate pretreatment technology for production of cellulase from rice straw

稻杆作为一种农业废弃物,其综合利用具有极大的社会经济价值。在本研究中,发现稻秆中可溶性多糖可以促进纤维素酶的生产,预处理对稻秆中可溶性多糖的促进作用影响巨大。糖组分分析表明碱制备多糖和水制备多糖的单糖组分有较大的差异。葡萄糖均是两种多糖的主要成分,但水制备多糖的葡萄糖含量较高,而碱制备多糖中的木糖含量是水制备的多糖中的5倍。碱制备多糖对纤维素酶的提高并不显著,尤其β-葡萄糖苷酶,而水制备多糖可显著提高β-葡萄糖苷酶活性。结果表明,通过耦合水热提取可溶性多糖工艺和碱处理工艺,滤纸酶活相比碱预处理提高了1. 2倍,β-葡萄糖苷酶活提高了2. 5倍。

As an agricultural waste,the utilization of rice straw has great social and economic value.In this study,it was found that the soluble polysaccharides derived from rice straw could promote the production of cellulase,and the pretreatment played an important role in affecting the promoting effect of the soluble saccharides on cellulase production.The sugar composition analysis showed that the monosaccharide components of the saccharides derived from alkali pretreatment and from water pretreatment were quite different.Glucose was the main component of both polysaccharides.Glucose content of the saccharides derived from water pretreatment was higher than that from alkali pretreatment,while xylose content of the saccharides derived from alkali pretreatment was 5 times higher than that from water pretreatment.The saccharides derived from alkali pretreatment had little effect on cellulase,especially onβ-glucosidase,while the saccharides derived from water pretreatment could significantly improve the activity ofβ-glucosidase.The results showed that by combining the water pretreatment and alkali pretreatment,the activity of FPase increased by 1.2 times when compared with alkali pretreatment alone,and the activity ofβ-glucosidase increased by 2.5 times.